지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제9호
- 발행연도
- 2009.1
- 수록면
- 888 - 897 (10page)
이용수
초록· 키워드
오류제보하기
Lipase diversity in glacier soil was assessed by cultureindependent
metagenomic DNA fragment screening and
confirmed by cell culture experiments. A set of degenerate
PCR primers specific for lipases of the hormone-sensitive
lipase family was designed based on conserved motifs and
used to directly PCR amplify metagenomic DNA from
glacier soil. These products were used to construct a lipase
fragment clone library. Among the 300 clones sequenced
for the analysis, 201 clones encoding partial lipases shared
51-82% identity to known lipases in GenBank. Based on
a phylogenetic analysis, five divergent clusters were
established, one of which may represent a previously
unidentified lipase subfamily. In the culture study, 11
lipase-producing bacteria were selectively isolated and
characterized by 16S rDNA sequences. Using the abovementioned
degenerate primers, seven lipase gene fragments
were cloned, but not all of them could be accounted for by
the clones in the library. Two full-length lipase genes
obtained by TAIL-PCR were expressed in Pichia pastoris
and characterized. Both were authentic lipases with
optimum temperatures of ≤40oC. Our study indicates the
abundant lipase diversity in glacier soil as well as the
feasibility of sequence-based screening in discovering new
lipase genes from complex environmental samples.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Lipase 유전자의 보존적 영역 탐색
생명과학회지
2003 .10
-
Gene Cloning, Purification, and Characterization of a Cold-Adapted Lipase Produced by Acinetobacter baumannii BD5
Journal of Microbiology and Biotechnology
2009 .01
-
Lipase-producing Filamentous Fungi from Non-dairy Creamer Industrial Waste
한국미생물·생명공학회지
2020 .01
-
Characterization of Lipases from Staphylococcus aureus and Staphylococcus epidermidis Isolated from Human Facial Sebaceous Skin
Journal of Microbiology and Biotechnology
2012 .01
-
Gene Cloning, High-Level Expression, and Characterization of an Alkaline and Thermostable Lipase from Trichosporon coremiiforme V3
Journal of Microbiology and Biotechnology
2015 .01
-
곤충 장내미생물로부터 lipase 생산능력이 우수한 Burkholderia sp. HY-10 균주의 분리 및 특성
한국응용곤충학회지
2007 .01
-
Isolation and Biochemical Characterization of Bacillus pumilus Lipases from the Antarctic
Journal of Microbiology and Biotechnology
2013 .01
-
Isolation and Characterization of a Novel Bacterium Burkholderia gladioli Bsp-1 Producing Alkaline Lipase
Journal of Microbiology and Biotechnology
2019 .01
-
미강 Lipase의 효소 화학적 성질에 대한 연구
한국영양학회지
1981 .09
-
A Direct Approach for Finding Functional Lipolytic Enzymes from the Paenibacillus polymyxa Genome
Journal of Microbiology and Biotechnology
2005 .01
-
메주로부터 지질분해 효소 생산 균주의 분리 및 배양학적 특성
한국미생물·생명공학회지
2012 .01
-
Potential Yeast from Indonesian Wild Forest Honey Showing Ability to Produce Lipase for Lipid Transesterification
한국미생물·생명공학회지
2019 .01
-
유류오염지역에서 분리한 Acinetobacter sp. B2로부터의 Lipase 정제 및 특성
미생물학회지
2004 .12
-
Isolation of Lipase Genes from Goat Ruminal Metagenomic Libraries
한국미생물학회 학술대회논문집
2016 .04
-
S5 Lipase : An Organic Solvent Tolerant Enzyme
The Journal of Microbiology
2006 .12
-
Optimization of Lipase Pretreatment Prior to Lipase Immobilization to Prevent Loss of Activity
Journal of Microbiology and Biotechnology
2007 .01
-
Purification and Characterization of a Novel Thermophilic Lipase from Acinetobacter sp. D12
한국생명과학회 심포지움
2008 .10
-
Lipase Production in Solid State Fermentation by Fungi Using Statistical Experimental Designs
한국미생물학회 학술대회논문집
2009 .05
이 논문의 저자 정보
최근 본 자료
댓글(0)
0