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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제19권 제9호
- 발행연도
- 2009.1
- 수록면
- 966 - 971 (6page)
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초록· 키워드
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Peroxidase from Coprinus cinereus (CiP) has attracted
attention for its high specific activity and broad substrate
spectrum compared with other peroxidases. In this study,
the functional expression of this peroxidase was successfully
achieved in the methylotrophic yeast Pichia pastoris. The
expression level of CiP was increased by varying the
microbial hosts and the expression promoters. Since a signal
sequence, such as the alpha mating factor of Saccharomyces
cerevisiae, was placed preceding the cDNA of the CiP coding
gene, expressed recombinant CiP (rCiP) was secreted into
the culture broth. The Mut+ Pichia pastoris host showed a
3-fold higher peroxidase activity, as well as 2-fold higher
growth rate, compared with the Muts Pichia pastoris host.
Furthermore, the AOX1 promoter facilitated a 5-fold higher
expression of rCiP than did the GAP promoter.
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참고문헌 (26)
참고문헌 신청
Abdelmoula, S. / 2007 / High-level expression of human tumor suppressor P53 in the methylotrophic yeast: Pichia pastoris / Protein Expr. Purif 54 : 283 ~ 288
Arnold, R. R. / 1980 / Bactericidal activity of human lactoferrin: Sensitivity of a variety of microorganisms / Infect. Immun 28 : 893 ~ 898
Arnold, R. R. / 1982 / Bactericidal activity of human lactoferrin: Differentiation from the stasis of iron deprivation / Infect. Immun 35 : 792 ~ 799
Bibila, T. / 1991 / A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells / Biotechnol. Bioeng 37 : 210 ~ 226
Boer, H. / 2000 / Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters / Biotechnol. Bioeng 69 : 486 ~ 4
Arnold, R. R. / 1980 / Bactericidal activity of human lactoferrin: Sensitivity of a variety of microorganisms / Infect. Immun 28 : 893 ~ 898
Arnold, R. R. / 1982 / Bactericidal activity of human lactoferrin: Differentiation from the stasis of iron deprivation / Infect. Immun 35 : 792 ~ 799
Bibila, T. / 1991 / A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells / Biotechnol. Bioeng 37 : 210 ~ 226
Boer, H. / 2000 / Characterization of Trichoderma reesei cellobiohydrolase Cel7A secreted from Pichia pastoris using two different promoters / Biotechnol. Bioeng 69 : 486 ~ 4
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