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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제1호
- 발행연도
- 2013.1
- 수록면
- 64 - 68 (5page)
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초록· 키워드
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Previous studies have shown that lactic acid bacteria can inhibit inflammatory responses, but the mechanisms are very little known. In this study, transaction and expression of three proinflammatory factors, iNOS, PTGS-2, and IL8,which are closely related to the inflammatory response,were investigated by luciferase reporter assay and RTPCR in HT-29 cells treated by Lactobacillus acidophilus.
The results showed that the live L. acidophilus sharply down-regulated the transcription of these three genes.
Because there was a NF-κB binding site located at -265 bp,-225 bp, and -95 bp upstream of the iNOS, PTGS-2, and IL8 promoters, respectively, we further addressed the effects of NF-κB on transaction of the three promoters by cotransfection. As was expected, NF-κBs remarkably upregulated the activity of the reporter gene and, no effect of NF-κBs on IL-8 promoter transaction was found after NF-κB binding site mutation of the IL8 promoter in HT-29 cells. In conclusion, the live L. acidophilus decreased the transcriptional activity of NF-κB and, in turn, inhibited the transaction of NF-κB on the three proinflammatory factors mentioned above.
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참고문헌 (25)
참고문헌 신청
Angulo, S / 2006 / Lactobacillus casei prevents the upregulation of ICAM-1 expression and leukocyte recruitment in experimental colitis. Am / J. Physiol. Gastrointest. Liver
Baeuerle, P. A / 1996 / NF-kappa B: ten years after / Cell 87 : 13 ~ 20
Bai, A. P / 2006 / Probiotics and inflammatory bowel diseases / Postgrad. Med. J 82 : 376 ~ 382
Beinke, S / 2004 / Functions of NF-κB1 and NFeB2 in immune cell biology / Biochem. J 382 : 393 ~ 409
Bharrhan, S / 2012 / Down-regulation of NF-κB signalling by polyphenolic compounds prevents endotoxin-induced liver injury in a rat model / Innate Immun 18 : 70 ~ 79
Baeuerle, P. A / 1996 / NF-kappa B: ten years after / Cell 87 : 13 ~ 20
Bai, A. P / 2006 / Probiotics and inflammatory bowel diseases / Postgrad. Med. J 82 : 376 ~ 382
Beinke, S / 2004 / Functions of NF-κB1 and NFeB2 in immune cell biology / Biochem. J 382 : 393 ~ 409
Bharrhan, S / 2012 / Down-regulation of NF-κB signalling by polyphenolic compounds prevents endotoxin-induced liver injury in a rat model / Innate Immun 18 : 70 ~ 79
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