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초록· 키워드

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Significant alteration in the microbial community can occur across reclamation areas sufferingsubsidence from mining. A reclamation site undergoing fertilization practices and an adjacentcoal-excavated subsidence site (sites A and B, respectively) were examined to characterize thebacterial diversity using 454 high-throughput 16S rDNA sequencing. The dominant taxonomicgroups in both the sites were Proteobacteria, Acidobacteria, Bacteroidetes, Betaproteobacteria,Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi,and Firmicutes. However, the bacterial communities’ abundance, diversity, and compositiondiffered significantly between the sites. Site A presented higher bacterial diversity and morecomplex community structures than site B. The majority of sequences related to Proteobacteria,Gemmatimonadetes, Chloroflexi, Nitrospirae, Firmicutes, Betaproteobacteria, Deltaproteobacteria,and Anaerolineae were from site A; whereas those related to Actinobacteria, Planctomycetes,Bacteroidetes, Verrucomicrobia, Gammaproteobacteria, Nitriliruptoria, Alphaproteobacteria,and Phycisphaerae originated from site B. The distribution of some bacterial groups andsubgroups in the two sites correlated with soil properties and vegetation due to reclamationpractice. Site A exhibited enriched bacterial community, soil organic matter (SOM), and totalnitrogen (TN), suggesting the presence of relatively diverse microorganisms. SOM and TN wereimportant factors shaping the underlying microbial communities. Furthermore, the specificplant functional group (legumes) was also an important factor influencing soil microbialcommunity composition. Thus, the effectiveness of 454 pyrosequencing in analyzing soilbacterial diversity was validated and an association between land ecological system restoration,mostly mediated by microbial communities, and an improvement in soil properties in coalminingreclamation areas was suggested.

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