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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제11호
- 발행연도
- 2014.1
- 수록면
- 1,484 - 1,489 (6page)
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초록· 키워드
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A novel esterase gene, est01, was successfully unearthed from a biogas digester microbiota metagenomic library. The 1,194 bp est01 gene encodes a protein of 44,804 Da (designated Est01). The amino acid sequence of Est01 shows only moderate (33%) identity to a lipase/ esterase. Phylogenetic analysis and biochemical characterization confirmed that Est01 is a new member of family VIII esterases. The purified Est01 from recombinant Escherichia coli BL21 (DE3) showed high hydrolytic activity against short-chain fatty acid esters, suggesting that it is a typical carboxylesterase rather than a lipase. Furthermore, the Est01 was even active at 10°C (43% activity remained), with the optimal temperature at 20°C, and had a broad pH range from 5.0 to 10.0, with the optimal pH of 8.0. These properties suggest that Est01 is a cold-adaptive esterase and could have good potential for low-temperature hydrolysis application.
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참고문헌 (23)
참고문헌 신청
Arpigny JL / 1999 / Bacterial lipolytic enzymes:classification and properties / Biochem J 343 : 177 ~ 183
Aurilia V / 2008 / Microbial carbohydrate esterases in cold adapted environments / Gene 410 : 234 ~ 240
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Cheng XJ / 2010 / Screening of microbial community in biogas fermentation under low temperature and construction of its metagenome library / China Biotechnol 30 : 50 ~ 55
Cheng XJ / 2011 / Subtilase genes diversity in the biogas digester microbiota / Curr. Microbiol 62 : 1542 ~ 1547
Aurilia V / 2008 / Microbial carbohydrate esterases in cold adapted environments / Gene 410 : 234 ~ 240
Bradford MM. / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 :
Cheng XJ / 2010 / Screening of microbial community in biogas fermentation under low temperature and construction of its metagenome library / China Biotechnol 30 : 50 ~ 55
Cheng XJ / 2011 / Subtilase genes diversity in the biogas digester microbiota / Curr. Microbiol 62 : 1542 ~ 1547
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