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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제1호
- 발행연도
- 2015.1
- 수록면
- 18 - 25 (8page)
이용수
초록· 키워드
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To understand the metabolism of flavonoid rhamnoglycosides by human intestinal microbiota, we measured the metabolic activity of rutin and poncirin (distributed in many functional foods and herbal medicine) by 100 human stool specimens. The average α-Lrhamnosidase activities on the p-nitrophenyl-α-L-rhamnopyranoside, rutin, and poncirin subtrates were 0.10 ± 0.07, 0.25 ± 0.08, and 0.15 ± 0.09 pmol/min/mg, respectively. To investigate the enzymatic properties, α-L-rhamnosidase-producing bacteria were isolated from the specimens, and the α-L-rhamnosidase gene was cloned from a selected organism, Bifidobacterium dentium, and expressed in E. coli. The cloned α-L-rhamnosidase gene contained a 2,673 bp sequcence encoding 890 amino acid residues. The cloned gene was expressed using the pET 26b(+) vector in E. coli BL21, and the expressed enzyme was purified using Ni2+-NTA and Q-HP column chromatography. The specific activity of the purified α-L-rhamnosidase was 23.3 μmol/min/mg. Of the tested natural product constituents, the cloned α-L-rhamnosidase hydrolyzed rutin most potently, followed by poncirin, naringin, and ginsenoside Re. However, it was unable to hydrolyze quercitrin. This is the first report describing the cloning, expression, and characterization of α-L-rhamnosidase, a flavonoid rhamnoglycosidemetabolizing enzyme, from bifidobacteria. Based on these findings, the α-L-rhamnosidase of intestinal bacteria such as B. dentium seem to be more effective in hydrolyzing (1→6) bonds than (1→2) bonds of rhamnoglycosides, and may play an important role in the metabolism and pharmacological effect of rhamnoglycosides.
목차
등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Avila M / 2009 / Physiological and biochemical characterization of the two alpha-L-rhamnosidases of Lactobacillus plantarum NCC245 / Microbiology 155 : 2739 ~ 2749
Beekwilder J / 2009 / Characterization of rhamnosidases from Lactobacillus plantarum and Lactobacillus acidophilus / Appl. Environ. Microbiol 75 : 3447 ~ 3454
Crow JM. / 2011 / Microbiome: that healthy gut feeling / Nature 480 : S88 ~ S89
Dai-SikLee / 2002 / Fecal Metabolic Activities of Herbal Components to Bioactive Compounds / Archives of Pharmacal Research 25 (3) : 165 ~ 169
De Filippo C / 2010 / Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa / Proc. Natl. Acad. Sci. USA 107 : 146
Beekwilder J / 2009 / Characterization of rhamnosidases from Lactobacillus plantarum and Lactobacillus acidophilus / Appl. Environ. Microbiol 75 : 3447 ~ 3454
Crow JM. / 2011 / Microbiome: that healthy gut feeling / Nature 480 : S88 ~ S89
Dai-SikLee / 2002 / Fecal Metabolic Activities of Herbal Components to Bioactive Compounds / Archives of Pharmacal Research 25 (3) : 165 ~ 169
De Filippo C / 2010 / Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa / Proc. Natl. Acad. Sci. USA 107 : 146
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