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논문 기본 정보
- 자료유형
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제25권 제1호
- 발행연도
- 2015.1
- 수록면
- 11 - 17 (7page)
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초록· 키워드
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In an attempt to shift the optimal pH of the xylanase B (XynB) from Aspergillus niger towards alkalinity, target mutation sites were selected by alignment between Aspergillus niger xylanase B and other xylanases that have alkalophilic pH optima that highlight charged residues in the eight-residues-longer loop in the alkalophilic xylanase. Multiple engineered XynB mutants were created by site-directed mutagenesis with substitutions Q164K and Q164K+D117N. The variant XynB-117 had the highest optimum pH (at 5.5), which corresponded to a basic 0.5 pH unit shift when compared with the wild-type enzyme. However, the optimal pH of the XynB- 164 mutation was not changed, similar to the wild type. These results suggest that the residues at positions 164 and 117 in the eight-residues-longer loop and the cleft’s edge are important in determining the pH optima of XynB from Aspergillus niger.
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참고문헌 (23)
참고문헌 신청
Ahmed S / 2009 / Molecular cloning of fungal xylanases: an overview / Appl. Microbiol. Biotechnol 84 : 19 ~ 35
Asghari SM / 2004 / Acid-induced conformational changes in Bacillus amyloliquefaciens alpha-amylase: appearance of a molten globule like state / Enzyme Microb. Technol 35 : 5
Baba T / 1994 / Identification and characterization of clustered genes for thermostable xylandegrading enzymes, β-xylosidase and xylanase of Bacillus stearothermophilus 21 /
Bardford, M. / 1976 / Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 : 2
Bergquist P / 2002 / Expression of xylanase enzymes from thermophilic microorganisms in fungal host / Extremophiles 6 : 177 ~ 184
Asghari SM / 2004 / Acid-induced conformational changes in Bacillus amyloliquefaciens alpha-amylase: appearance of a molten globule like state / Enzyme Microb. Technol 35 : 5
Baba T / 1994 / Identification and characterization of clustered genes for thermostable xylandegrading enzymes, β-xylosidase and xylanase of Bacillus stearothermophilus 21 /
Bardford, M. / 1976 / Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72 : 2
Bergquist P / 2002 / Expression of xylanase enzymes from thermophilic microorganisms in fungal host / Extremophiles 6 : 177 ~ 184
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