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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 271 - 280 (10page)
이용수
초록· 키워드
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Vibrio alginolyticus, a Gram-negative marine bacterium,is one of the causative agents of fish vibriosis. Its virulence factors and pathogenesis mechanism are barely known,except for some extracellular products (ECPs) that are known to be regulated by quorum sensing system.
Therefore, the present study used a microarray to analyze the transcription profiles of the wild-type V. alginolyticus and a deletion mutant of luxO, the pivotal regulator in Vibrio quorum sensing systems, which resulted in the identification of a putative virulence factor, MviN.
Quantitative real-time reverse transcription PCR confirmed that the transcription of mviN was upregulated in the luxO mutant when compared with wild-type, and downregulated in a luxO-con complemented strain. Furthermore, Western blotting indicated that MviN was greatly induced during the late-exponential and stationary phases of growth,indicating that the expression of MviN was cell-density dependent and quorum sensing regulated in V. alginolyticus.
Meanwhile, the mviN null mutant displayed a much slower growth rate than the wild type, signifying the essential role of MviN in V. alginolyticus. Western blotting also revealed that MviN was present as an extracellular protein in V. alginolyticus. When epithelioma papulosum cyprini (EPC) cells were treated with the ECPs of the mviN mutant, no cytotoxicity was observed, whereas EPC cells treated with the wild type exhibited pathological changes, which increased with the ECPs concentration and treatment time. Therefore, the results demonstrated that MviN is a LuxO-regulated ECPs component and involved in the pathogenicity of V. alginolyticus.
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참고문헌 (21)
참고문헌 신청
Aguirre-Guzman, G / 2004 / A review of extracellular virulence product of vibrio species important in diseases of cultivated shrimp / Aquac. Res 35 : 1395 ~ 1404
Balebona, M. C / 1998 / Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.) / Appl. Environ. Microbiol 64 : 4269 ~ 4275
Cai, S. H / 2007 / Cloning and expression of gene encoding the thermostable direct hemolysin from Vibrio alginolyticus strain HY9901, the causative agent of vibriosis of crim
Cai, S. H / 2007 / Cloning and expression of the gene encoding an extracellular alkaline serine protease from Vibrio alginolyticus strain HY9901, the causative agent of vibri
Carsiotis, M / 1989 / A Salmonella typhimurium virulence gene linked to flg / Infect. Immun 57 : 3276 ~ 3280
Balebona, M. C / 1998 / Pathogenicity of Vibrio alginolyticus for cultured gilt-head sea bream (Sparus aurata L.) / Appl. Environ. Microbiol 64 : 4269 ~ 4275
Cai, S. H / 2007 / Cloning and expression of gene encoding the thermostable direct hemolysin from Vibrio alginolyticus strain HY9901, the causative agent of vibriosis of crim
Cai, S. H / 2007 / Cloning and expression of the gene encoding an extracellular alkaline serine protease from Vibrio alginolyticus strain HY9901, the causative agent of vibri
Carsiotis, M / 1989 / A Salmonella typhimurium virulence gene linked to flg / Infect. Immun 57 : 3276 ~ 3280
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