지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제7호
- 발행연도
- 2012.1
- 수록면
- 1,021 - 1,028 (8page)
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초록· 키워드
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In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid, sensitive, and inexpensive detection of nervous necrosis virus (NNV) in olive flounder, Paralichthys olivaceus, in Korea. A set of six specific primers was designed to target the RNA 2 gene encoding the coat protein of Korean NNV strains. The RT-LAMP reaction successfully detected NNV after 30 min at 65oC. When the sensitivities among RT-LAMP, RT-PCR, and nested RTPCR were compared, the RT-LAMP was shown to be able to detect the RNA template at 2.58 × 10-2 TCID50/ml,whereas the RT-PCR and nested RT-PCR were only able to detect the RNA template at 2.58 × 102 TCID50/ml and 2.58 TCID50/ml, respectively. Thus, the sensitivity of the RT-LAMP assay was higher than those of the RT-PCR assays. In the specificity test of the RT-LAMP, 2 genotypes of NNVs (SJNNV and RGNNV) were positive; however,no other fish viruses were positive with the primers,indicating that the RT-LAMP assay is only specific to NNV. A total of 102 olive flounder were collected from hatcheries between 2009 and 2011. The occurrence of NNV in olive flounder was determined to be 53.9% (55/102) by the RT-LAMP. On the other hand, the prevalence based on the nested RT-PCR and RT-PCR results was 33.8% (34/102) and 20.6% (21/102), respectively. This result indicates that the RT-LAMP assay developed in this study is suitable for early field diagnosis of NNV with high sensitivity.
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등록된 정보가 없습니다.
참고문헌 (23)
참고문헌 신청
Arimoto, M. / 1992 / Detection of striped jack nervous necrosis virus (SJNNV) by enzyme-linked immunosorbent assay (ELISA) / Gyobyo Kenkyu 27 : 191 ~ 195
Breuil, G. / 1991 / Viral infection (picorna-like virus) associated with mass mortalities in the hatchery-reared sea-bass (Dicentrarchus labrax) larvae and juveniles / Aquacu
Cha, S. J. / 2007 / Phylogenetic analysis of betanodaviruses isolated from cultured fish in Korea / DISEASES OF AQUATIC ORGANISMS 77 (3) : 181 ~ 189
Chi, S. C. / 1997 / Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus
Dalla Valle, L. / 2005 / Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR / Vet. Microbiol 110 :
Breuil, G. / 1991 / Viral infection (picorna-like virus) associated with mass mortalities in the hatchery-reared sea-bass (Dicentrarchus labrax) larvae and juveniles / Aquacu
Cha, S. J. / 2007 / Phylogenetic analysis of betanodaviruses isolated from cultured fish in Korea / DISEASES OF AQUATIC ORGANISMS 77 (3) : 181 ~ 189
Chi, S. C. / 1997 / Mass mortalities associated with viral nervous necrosis (VNN) disease in two species of hatchery-reared grouper, Epinephelus fuscogutatus and Epinephelus
Dalla Valle, L. / 2005 / Development of a sensitive and quantitative diagnostic assay for fish nervous necrosis virus based on two-target real-time PCR / Vet. Microbiol 110 :
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