지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제9호
- 발행연도
- 2012.1
- 수록면
- 1,230 - 1,236 (7page)
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초록· 키워드
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Fish meal grades SL1 and SL2 from Sardine (Sardinella longiceps) and NJ from Pink Perch (Nemipterus japonicas)were evaluated as a sole source of carbon and nitrogen in the medium for alkaline protease production by Bacillus pumilus MTCC 7514. The analysis of the fish meal suggests that the carbon and nitrogen contents in fish meal are sufficient to justify its choice as replacement for other nutrients. Protease production increased significantly (4,914 U/ml) in medium containing only fish meal,compared with the basal medium (2,646 U/ml). However,the elimination of inorganic salts from media reduced the protease productivity. In addition, all the three grades of fish meal yielded almost the same amounts of protease when employed as the sole source of carbon and nitrogen.
Nevertheless, the best results were observed in fish meal SL1 medium. Furthermore, protease production was enhanced to 6,966 U/ml and 7,047 U/ml on scaling up from flask (4,914 U/ml) to 3.7 and 20 L fermenters, respectively,using fish meal (10 g/l). Similarly, the corresponding improvement in productivities over flask (102.38 U/ml/h)was 193.5 and 195.75 U/ml/h in 3.7 and 20 L fermenters,respectively. The crude protease was found to have dehairing ability in leather processing, which is bound to have great environmental benefits.
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참고문헌 (17)
참고문헌 신청
Blanco, M. / 2007 / Towards sustainable and efficient use of fishery resources:Present and future trends / Trends Food Sci. Technol 18 : 29 ~ 36
Ellouz, Y. / 2001 / Production of protease by Bacillus subtilis grown on sardinelle heads and viscera flour / Bioresour. Technol 80 : 49 ~ 51
Ellouz, Y. T. / 2003 / Biosynthesis of protease by Pseudomonas aeruginosa MN7 grown on fish substrate / World J. Microbiol. Biotechnol 19 : 41 ~ 45
Hadj-Ali, N. E. / 2010 / The use of an economical medium for the production of alkaline serine proteases by Bacillus licheniformis NH1 / Afr. J. Biotechnol 9 : 2668 ~ 2674
Huang, Q. / 2003 / Purification and characterization of an extracellular alkaline serine protease with dehairing function from Bacillus pumilus / Curr. Microbiol 46 : 169 ~ 1
Ellouz, Y. / 2001 / Production of protease by Bacillus subtilis grown on sardinelle heads and viscera flour / Bioresour. Technol 80 : 49 ~ 51
Ellouz, Y. T. / 2003 / Biosynthesis of protease by Pseudomonas aeruginosa MN7 grown on fish substrate / World J. Microbiol. Biotechnol 19 : 41 ~ 45
Hadj-Ali, N. E. / 2010 / The use of an economical medium for the production of alkaline serine proteases by Bacillus licheniformis NH1 / Afr. J. Biotechnol 9 : 2668 ~ 2674
Huang, Q. / 2003 / Purification and characterization of an extracellular alkaline serine protease with dehairing function from Bacillus pumilus / Curr. Microbiol 46 : 169 ~ 1
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