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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제9호
- 발행연도
- 2012.1
- 수록면
- 1,224 - 1,229 (6page)
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초록· 키워드
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Puerarin (P), an isoflavone derived from kudzu roots, has strong biological activities, but its bioavailability is often limited by its low water solubility. To increase its solubility,P was glucosylated by three dextransucrases from Leuconostoc or Streptococcus species. Leuconostoc lactis EG001 dextransucrase exhibited the highest productivity of puerarin glucosides (P-Gs) among the three tested enzymes, and it primarily produced two P-Gs with a 53%yield. Their structures were identified as α-D-glucosyl-(1→6)-P (P-G) by using LC-MS or 1H- or 13C-NMR spectroscopies and α-D-isomaltosyl-(1→6)-P (P-IG2) by using specific enzymatic hydrolysis, and their solubilities were 15- and 202-fold higher than that of P, respectively.
P-G and P-IG2 are easily applicable in the food and pharmaceutical industries as alternative functional materials.
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참고문헌 (21)
참고문헌 신청
Ashok K. Singh / 2004 / Effects of Purified Puerarin on Voluntary Alcohol Intake and Alcohol Withdrawal Symptoms in P Rats Receiving Free Access to Water and Alcohol / Journa
Boue, S. M. / 2003 / Evaluation of the estrogenic effects of legume extracts containing phytoestrogens / J. Agric. Food Chem 51 : 2193 ~ 2199
Bradford, M. M. / 1976 / Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Choi, C. H. / 2010 / Enzymatic synthesis of glycosylated puerarin using maltogenic amylase from Bacillus stearothermophilus expressed in Bacillus subtilis / JOURNAL OF THE SC
Chung, H. J. / 2009 / Toxicological evaluation of the isoflavone puerarin and its glycosides / EUROPEAN FOOD RESEARCH AND TECHNOLOGY 230 : 145 ~ 153
Boue, S. M. / 2003 / Evaluation of the estrogenic effects of legume extracts containing phytoestrogens / J. Agric. Food Chem 51 : 2193 ~ 2199
Bradford, M. M. / 1976 / Rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal. Biochem 72
Choi, C. H. / 2010 / Enzymatic synthesis of glycosylated puerarin using maltogenic amylase from Bacillus stearothermophilus expressed in Bacillus subtilis / JOURNAL OF THE SC
Chung, H. J. / 2009 / Toxicological evaluation of the isoflavone puerarin and its glycosides / EUROPEAN FOOD RESEARCH AND TECHNOLOGY 230 : 145 ~ 153
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