지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제9호
- 발행연도
- 2012.1
- 수록면
- 1,301 - 1,306 (6page)
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초록· 키워드
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We combined real-time RT-PCR and real-time PCR (R/P)assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 nonrespiratory and 207 respiratory specimens from 218patients. Total nucleic acids (including RNA and DNA)were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ≤35 and the ratio of real-time RT-PCR and real-time PCR load was ≥1.51. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity,specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including nonrespiratory and respiratory specimens) were 98.2%, 97.2%,91.7%, and 99.4%, respectively, for R/P assay, and 83.9%,89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC,which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.
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참고문헌 (22)
참고문헌 신청
Al Zahrani, K. / 2000 / Accuracy and utility of commercially available amplification and serologic tests for the diagnosis of minimal pulmonary tuberculosis / Am. J. Respir.
Araj, G. / 2000 / Comparative performance of PCR-based assay versusmicroscopy and culture for the direct detection of Mycobacterium tuberculosis in clinical respiratory speci
Broccolo, F. / 2003 / Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays / J. Clin. Microb
Bustin, S. A. / 2009 / The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments / Clin. Chem 55 : 611 ~ 622
Cloud, J. / 2002 / Identification of Mycobacterium spp. by using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries / J. Clin. Microbiol 40 : 4
Araj, G. / 2000 / Comparative performance of PCR-based assay versusmicroscopy and culture for the direct detection of Mycobacterium tuberculosis in clinical respiratory speci
Broccolo, F. / 2003 / Rapid diagnosis of mycobacterial infections and quantitation of Mycobacterium tuberculosis load by two real-time calibrated PCR assays / J. Clin. Microb
Bustin, S. A. / 2009 / The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments / Clin. Chem 55 : 611 ~ 622
Cloud, J. / 2002 / Identification of Mycobacterium spp. by using a commercial 16S ribosomal DNA sequencing kit and additional sequencing libraries / J. Clin. Microbiol 40 : 4
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