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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제8호
- 발행연도
- 2013.1
- 수록면
- 1,060 - 1,069 (10page)
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초록· 키워드
오류제보하기
Genome organization near cyclomaltodextrinases (CDases) was analyzed and compared for four different hyperthermophilic archaea: Thermococcus, Pyrococcus, Staphylothermus, and Thermofilum. A gene (CL1_0884) encoding a putative CDase from Thermococcus sp. CL1 (tccd)was cloned and expressed in Escherichia coli. TcCD was confirmed to be highly thermostable,with optimal activity at 85oC. The melting temperature of TcCD was determined to be 93oC by both differential scanning calorimetry and differential scanning fluorimetry. A size-exclusion chromatography experiment showed that TcCD exists as a monomer. TcCD preferentially hydrolyzed α-cyclodextrin (α-CD), and at the initial stage catalyzed a ring-opening reaction by cleaving one α-1,4-glycosidic linkage of the CD ring to produce the corresponding single maltooligosaccharide. Furthermore, TcCD could hydrolyze branched CDs (G1-α-CD, G1-β-CD, and G2-β-CD) to yield significant amounts (45%, 40%, and 46%) of isomaltooligosaccharides (panose and 62-α-maltosylmaltose) in addition to glucose and maltose. This enzyme is one of the most thermostable maltogenic amylases reported, and might be of potential value in the production of isomaltooligosaccharides in the food industry.
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참고문헌 (21)
참고문헌 신청
Hashimoto Y / 2001 / Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. str
Holden JF / 2001 / Diversity among three novel groups of hyperthermophilic deep-sea Thermococcus species from three sites in the northeastern Pacific Ocean / FEMS Microbiol.
Jun X / 2012 / Complete genome sequence of the obligate piezophilic hyperthermophilic archaeon Pyrococcus yayanosii CH1 / J. Bacteriol 193 : 4297 ~ 4298
Jung JH / 2012 / Complete genome sequence of the hyperthermophilic archaeon Pyrococcus sp. strain ST04, isolated from a deepsea hydrothermal sulfide chimney on the Juan de Fu
Jung JH / 2012 / Complete genome sequence of the hyperthermophilic archaeon Thermococcus sp. strain CL1, isolated from a Paralvinella sp. polychaete worm collected from a hyd
Holden JF / 2001 / Diversity among three novel groups of hyperthermophilic deep-sea Thermococcus species from three sites in the northeastern Pacific Ocean / FEMS Microbiol.
Jun X / 2012 / Complete genome sequence of the obligate piezophilic hyperthermophilic archaeon Pyrococcus yayanosii CH1 / J. Bacteriol 193 : 4297 ~ 4298
Jung JH / 2012 / Complete genome sequence of the hyperthermophilic archaeon Pyrococcus sp. strain ST04, isolated from a deepsea hydrothermal sulfide chimney on the Juan de Fu
Jung JH / 2012 / Complete genome sequence of the hyperthermophilic archaeon Thermococcus sp. strain CL1, isolated from a Paralvinella sp. polychaete worm collected from a hyd
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