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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제23권 제3호
- 발행연도
- 2013.1
- 수록면
- 357 - 363 (7page)
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초록· 키워드
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The identification of bacteriophage proteins on the surface of Lactobacillus rhamnosus Pen was performed by LC-MS/MS analysis. Among the identified proteins, we found a phage-derived major tail protein, two major head proteins, a portal protein, and a host specificity protein.
Electron microscopy of a cell surface extract revealed the presence of phage particles in the analyzed samples. The partial sequence of genes encoding the major tail protein for all tested L. rhamnosus strains was determined with specific primers designed in this study. Next, RT-PCR analysis allowed detection of the expression of the major tail protein gene in L. rhamnosus strain Pen at all stages of bacterial growth. The transcription of genes encoding the major tail protein was also proved for other L. rhamnosus strains used in this study. The present work demonstrates the spontanous release of prophage-encoded particles by a commercial probiotic L. rhamnosus strain, which did not significantly affect the bacterial growth of the analyzed strain.
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참고문헌 (23)
참고문헌 신청
Ackermann, H. W / 2007 / 5500 phages in the electron microscope / Arch. Virol 152 : 227 ~ 243
Brandt, K / 2003 / Specific identification of certain probiotic Lactobacillus rhamnosus strains with PCR primers based on phage-related sequences / Int. J. Food Microbiol 84
Canchaya, C / 2004 / The impact of prophages on bacterial chromosomes / Mol. Microbiol 53 : 9 ~ 18
Capra, M. L / 2006 / Phages of Lactobacillus casei/paracasei: Response to environmental factors and interaction with collection and commercial strains / J. Appl. Microbiol 10
Casjens, S / 2003 / Prophages and bacterial genomics: What have we learned so far? / Mol. Microbiol 49 : 277 ~ 300
Brandt, K / 2003 / Specific identification of certain probiotic Lactobacillus rhamnosus strains with PCR primers based on phage-related sequences / Int. J. Food Microbiol 84
Canchaya, C / 2004 / The impact of prophages on bacterial chromosomes / Mol. Microbiol 53 : 9 ~ 18
Capra, M. L / 2006 / Phages of Lactobacillus casei/paracasei: Response to environmental factors and interaction with collection and commercial strains / J. Appl. Microbiol 10
Casjens, S / 2003 / Prophages and bacterial genomics: What have we learned so far? / Mol. Microbiol 49 : 277 ~ 300
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