지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제2호
- 발행연도
- 2014.1
- 수록면
- 160 - 167 (8page)
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초록· 키워드
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Oligopeptidase B (OpdB) is a serine peptidase widespread among bacteria and protozoa thathas emerged as a virulence factor despite its function has not yet been precisely established.
By using an OpdB-overexpressing Escherichia coli strain, we found that the overexpressedpeptidase makes the bacterial cells specifically less susceptible to several proline-richantimicrobial peptides known to penetrate into the bacterial cytosol, and that its level ofactivity directly correlates with the degree of resistance. We established that E. coli OpdB canefficiently hydrolyze in vitro cationic antimicrobial peptides up to 30 residues in length, eventhough they contained several prolines, shortening them to inactive fragments. Twoconsecutive basic residues are a preferred cleavage site for the peptidase. In the case of a singlebasic residue, there is no cleavage if proline residues are present in the P1 and P2 positions.
These results also indicate that cytosolic peptidases may cause resistance to antimicrobialpeptides that have an intracellular mechanism of action, such as the proline-rich peptides, andmay contribute to define the substrate specificity of the E. coli OpdB.
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참고문헌 (23)
참고문헌 신청
Benincasa M / 2004 / Antimicrobial activity of Bac7 fragments against drug-resistant clinical isolates / Peptides 25 : 2055 ~ 2061
Benincasa M / 2009 / Activity of antimicrobial peptides in the presence of polysaccharides produced by pulmonary pathogens / J. Pept. Sci 15 : 595 ~ 600
Brogden KA. / 2005 / Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? / Nat. Rev. Microbiol 3 : 238 ~ 250
Burleigh BA / 1997 / A cytosolic serine endopeptidase from Trypanosoma cruzi is required for the generation of Ca2+ signaling in mammalian cells / J. Cell Biol 136 : 609 ~ 62
Caler EV / 1998 / Oligopeptidase B-dependent signaling mediates host cell invasion by Trypanosoma cruzi / EMBO J 17 : 4975 ~ 4986
Benincasa M / 2009 / Activity of antimicrobial peptides in the presence of polysaccharides produced by pulmonary pathogens / J. Pept. Sci 15 : 595 ~ 600
Brogden KA. / 2005 / Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria? / Nat. Rev. Microbiol 3 : 238 ~ 250
Burleigh BA / 1997 / A cytosolic serine endopeptidase from Trypanosoma cruzi is required for the generation of Ca2+ signaling in mammalian cells / J. Cell Biol 136 : 609 ~ 62
Caler EV / 1998 / Oligopeptidase B-dependent signaling mediates host cell invasion by Trypanosoma cruzi / EMBO J 17 : 4975 ~ 4986
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