지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제5호
- 발행연도
- 2014.1
- 수록면
- 719 - 723 (5page)
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초록· 키워드
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Caspases are a family of cysteine proteases that play an important role in the apoptotic pathway. Caspase-6 is an apoptosis effector that cleaves a variety of cellular substrates. The active form of the enzyme is required for use in research. However, it has been difficult to obtain sufficient quantities of active caspase-6 from Escherichia coli. In the present study, we constructed a caspase-6 with a 23-amino-acid deletion in the pro-domain. This engineered enzyme was expressed as a soluble protein in E. coli and was purified using affinity resin. In vitro enzyme assay and cleavage analysis revealed that the engineered active caspase-6 protein had characteristics similar to those of wild-type caspase-6. This novel method can be a valuable tool for obtaining active caspase-6 that can be used for screening caspase-6–specific substrates, which in turn can be used to elucidate the function of caspase-6 in apoptosis.
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참고문헌 (15)
참고문헌 신청
Brown JM / 2005 / The role of apoptosis in cancer development and treatment response / Nat. Rev. Cancer 5 : 231 ~ 237
Ekici OD / 2006 / Design, synthesis, and evaluation of azapeptide Michael acceptors as selective and potent inhibitors of caspases-2, -3, -6, -7, -8, -9, and -10 / J. Med. Ch
Lamkanfi M / 2002 / Alice in caspase land. A phylogenetic analysis of caspases from worm to man / Cell Death Differ 9 : 358 ~ 361
Lazebnik YA / 1994 / Cleavage of poly(ADP-ribose)polymerase by a proteinase with properties like ICE / Nature 371 : 346 ~ 347
Lee AY / 2004 / Identification of caspase-3 degradome by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight analysis / Proteom
Ekici OD / 2006 / Design, synthesis, and evaluation of azapeptide Michael acceptors as selective and potent inhibitors of caspases-2, -3, -6, -7, -8, -9, and -10 / J. Med. Ch
Lamkanfi M / 2002 / Alice in caspase land. A phylogenetic analysis of caspases from worm to man / Cell Death Differ 9 : 358 ~ 361
Lazebnik YA / 1994 / Cleavage of poly(ADP-ribose)polymerase by a proteinase with properties like ICE / Nature 371 : 346 ~ 347
Lee AY / 2004 / Identification of caspase-3 degradome by two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight analysis / Proteom
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