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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제27권 제9호
- 발행연도
- 2017.1
- 수록면
- 1,559 - 1,565 (7page)
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초록· 키워드
오류제보하기
Naturally occurring ginsenoside F1 (20-O-β-D-glucopyranosyl-20(S)-protopanaxatriol) is rare.
Here, we produced gram-scale quantities of ginsenoside F1 from a crude protopanaxatriol saponin mixture comprised mainly of Re and Rg1 through enzyme-mediated biotransformation using recombinant β-glucosidase (BgpA) cloned from a soil bacterium, Terrabacter ginsenosidimutans Gsoil 3082T. In a systematic step-by-step process, the concentrations of substrate, enzyme, and NaCl were determined for maximal production of F1. At an optimized NaCl concentration of 200 mM, the protopanaxatriol saponin mixture (25 mg/ml) was incubated with recombinant BgpA (20 mg/ml) for 3 days in a 2.4 L reaction. Following octadecylsilyl silica gel column chromatography, 9.6 g of F1 was obtained from 60 g of substrate mixture at 95% purity, as assessed by chromatography. These results represent the first report of gramscale F1 production via recombinant enzyme-mediated biotransformation.
목차
등록된 정보가 없습니다.
참고문헌 (24)
참고문헌 신청
Minkyung Bae / 2014 / Protective effect of Korean Red Ginseng extract against Helicobacter pylori-induced gastric inflammation in Mongolian gerbils / Journal of Ginseng Research 38(1):8~15
이창호 / 2014 / A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases / Journal of Ginseng Research 38(3):161~166
Ho Jae Park / 2012 / Ginseng in the traditional herbal prescriptions / Journal of Ginseng Research 36(3):225~241
Christensen LP. / 2008 / Ginsenosides. Chemistry, biosynthesis, analysis, and potential health effects / Adv. Food Nutr. Res 55:1~99
Corbit RM / 2005 / Simplified extraction of ginsenosides from American ginseng (Panax quinquefolius L.) for high-performance liquid chromatographyultraviolet analysis / J. Agric. Food Chem 53:9867~9873
이창호 / 2014 / A review on the medicinal potentials of ginseng and ginsenosides on cardiovascular diseases / Journal of Ginseng Research 38(3):161~166
Ho Jae Park / 2012 / Ginseng in the traditional herbal prescriptions / Journal of Ginseng Research 36(3):225~241
Christensen LP. / 2008 / Ginsenosides. Chemistry, biosynthesis, analysis, and potential health effects / Adv. Food Nutr. Res 55:1~99
Corbit RM / 2005 / Simplified extraction of ginsenosides from American ginseng (Panax quinquefolius L.) for high-performance liquid chromatographyultraviolet analysis / J. Agric. Food Chem 53:9867~9873
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