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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제28권 제9호
- 발행연도
- 2018.1
- 수록면
- 1,547 - 1,553 (7page)
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초록· 키워드
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Hyaluronidases are a family of enzymes that catalyse the breakdown of hyaluronic acid, which is abundant in the extracellular matrix and cumulus oocyte complex. To investigate the activity of recombinant bovine sperm hyaluronidase 1 (SPAM1) and determine the effect of the Asn-X-Ser/Thr motif on its activity, the bovine SPAM1 open reading frame was cloned into the mammalian expression vector pCXN2 and then transfected to the HEK293 cell line. Expression of recombinant bovine hyaluronidase was estimated using a hyaluronidase activity assay with gel electrophoresis. Recombinant hyaluronidase could resolve highly polymeric hyaluronic acid and also caused dispersal of the cumulus cell layer. Comparative analysis with respect to enzyme activity was carried out for the glycosylated and deglycosylated bovine sperm hyaluronidase by N-glycosidase F treatment. Finally, mutagenesis analysis revealed that among the five potential N-linked glycosylation sites, only three contributed to significant inhibition of hyaluronic activity. Recombinant bovine SPAM1 has hyaluronan degradation and cumulus oocyte complex dispersion ability, and the N-linked oligosaccharides are important for enzyme activity, providing a foundation for the commercialization of hyaluronidase.
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참고문헌 (20)
참고문헌 신청
Zhu Y / 2018 / Hyaluronan in adipogenesis, adipose tissue physiology and systemic metabolism / Matrix Biol.
Kim E / 2013 / Molecular cloning, characterization of porcine IZUMO1, an IgSF family member / Reprod. Domest Anim. 48:90~97
Viola M / 2015 / Regulated hyaluronan synthesis by vascular cells / Int. J. Cell Biol. 2015:208303
Aya KL / 2014 / Hyaluronan in wound healing: rediscovering a major player / Wound Repair Regen. 22:579~593
Nguyen N / 2017 / Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities / Cell Commun. Signal. 15:48
Kim E / 2013 / Molecular cloning, characterization of porcine IZUMO1, an IgSF family member / Reprod. Domest Anim. 48:90~97
Viola M / 2015 / Regulated hyaluronan synthesis by vascular cells / Int. J. Cell Biol. 2015:208303
Aya KL / 2014 / Hyaluronan in wound healing: rediscovering a major player / Wound Repair Regen. 22:579~593
Nguyen N / 2017 / Human hyaluronic acid synthase-1 promotes malignant transformation via epithelial-to-mesenchymal transition, micronucleation and centrosome abnormalities / Cell Commun. Signal. 15:48
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