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- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제22권 제12호
- 발행연도
- 2012.1
- 수록면
- 1,621 - 1,628 (8page)
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The agar-degrading bacterium GNUM-1 was isolated from the brown algal species Sargassum serratifolium,which was obtained from the West Sea of Korea, by using the selective artificial seawater agar plate. The cells were Gram-negative, 0.5-0.6 μm wide and 2.0-2.5 μm long curved rods with a single polar flagellum, forming nonpigmented,circular, smooth colonies. Cells grew at 20oC-37oC, between pH 5.0 and 9.0, and at 1-10% (w/v) NaCl.
The DNA G+C content of the GNUM-1 strain was 45.5mol%. The 16S rRNA sequence of the GNUM-1 was very similar to those of Alteromonas stellipolaris LMG 21861(99.86% sequence homology) and Alteromonas addita R10SW13T(99.64% sequence homology), which led us to assign it to the genus Alteromonas. It showed positive activities for agarase, amylase, gelatinase, alkaline phosphatase,esterase (C8), lipase (C14), leucine arylamidase, valine arylamidase, α-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, α-galactosidase, β-galactosidase,β-glucosidase, catalase, and urease. It can utilize citrate,malic acid, and trisodium citrate. The major fatty acids were summed feature 3 (21.5%, comprising C16:1ω7c/iso-C15:0 2-OH) and C16:0 (15.04%). On the basis of the variations in many biochemical characteristics, GNUM-1was considered as unique and thus was named Alteromonas sp. GNUM-1. It produced the highest agarase activity in modified ASW medium containing 0.4% sucrose, but lower activity in rich media despite superior growth,implying that agarase production is tightly regulated and repressed in a rich nutrient condition. The 30 kDa protein with agarase activity was identified by zymography, and this report serves as the very first account of such a protein in the genus Alteromonas.
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참고문헌 (25)
참고문헌 신청
Altschul, S. F. / 1997 / Gapped BLAST and PSI-BLAST: A new generation of protein data base / Nucleic Acids Res 25 : 3389 ~ 3402
Araki, C. H. / 1937 / Acetylation of agar like substance of Gelidium amansii / J. Chem. Soc 58 : 1338 ~ 1350
Chen, Y. G. / 2009 / Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone / Antonie Van Leeuwenhoek 96 : 259 ~ 366
Chi, W. J. / 2012 / Agar degradation by microorganisms and agar-degrading enzymes / APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 94 (4) : 917 ~ 930
Chiu, H. H. / 2007 / Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary / Int. J. Syst. Evol. Microbio
Araki, C. H. / 1937 / Acetylation of agar like substance of Gelidium amansii / J. Chem. Soc 58 : 1338 ~ 1350
Chen, Y. G. / 2009 / Alteromonas halophila sp. nov., a new moderately halophilic bacterium isolated from a sea anemone / Antonie Van Leeuwenhoek 96 : 259 ~ 366
Chi, W. J. / 2012 / Agar degradation by microorganisms and agar-degrading enzymes / APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 94 (4) : 917 ~ 930
Chiu, H. H. / 2007 / Alteromonas tagae sp. nov. and Alteromonas simiduii sp. nov., mercury-resistant bacteria isolated from a Taiwanese estuary / Int. J. Syst. Evol. Microbio
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