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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제4호
- 발행연도
- 2014.1
- 수록면
- 431 - 439 (9page)
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We report the construction of two Bacillus subtilis expression vectors, pBNS1/pBNS2. Bothvectors are based on the strong promoter P43 and the ampicillin resistance gene expressioncassette. Additionally, a fragment with the Shine-Dalgarno sequence and a multiple cloningsite (BamHI, SalI, SacI, XhoI, PstI, SphI) were inserted. The coding region for the amyQ(encoding an amylase) signal peptide was fused to the promoter P43 of pBNS1 to construct thesecreted expression vector pBNS2. The applicability of vectors was tested by first generatingthe expression vectors pBNS1-GFP/pBNS2-GFP and then detecting for green fluorescentprotein gene expression. Next, the mannanase gene from B. pumilus Nsic-2 was fused to vectorpBNS2 and we measured the mannanase activity in the supernatant. The mannanase totalenzyme activity was 8.65 U/ml, which was 6 times higher than that of the parent strain. Ourwork provides a feasible way to achieve an effective transformation system for geneexpression in B. subtilis and is the first report to achieve B. pumilus mannanase secretoryexpression in B. subtilis.
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참고문헌 (18)
참고문헌 신청
Bisicchia P / 2010 / Suite of novel vectors for ectopic insertion of GFP, CFP and IYFP transcriptional fusions in single copy at the amyE and bglS loci in Bacillus subtilis /
Chen X / 2009 / Novel expression vector for secretion of cecropin AD in Bacillus subtilis with enhanced antimicrobial activity / Antimicrob. Agents Chemother 53 : 3683 ~ 3689
Dhawan S / 2007 / Microbial mannanases: an overview of production and applications / Crit. Rev. Biotechnol 27 : 197 ~ 216
Hartl B / 2001 / Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes / J. Bacteriol 183 : 2696 ~
Li YN / 2006 / A beta-mannanase from Bacillus subtilis B36: purification, properties, sequencing, gene cloning and expression in Escherichia coli / Z. Naturforsch 61 : 840 ~
Chen X / 2009 / Novel expression vector for secretion of cecropin AD in Bacillus subtilis with enhanced antimicrobial activity / Antimicrob. Agents Chemother 53 : 3683 ~ 3689
Dhawan S / 2007 / Microbial mannanases: an overview of production and applications / Crit. Rev. Biotechnol 27 : 197 ~ 216
Hartl B / 2001 / Development of a new integration site within the Bacillus subtilis chromosome and construction of compatible expression cassettes / J. Bacteriol 183 : 2696 ~
Li YN / 2006 / A beta-mannanase from Bacillus subtilis B36: purification, properties, sequencing, gene cloning and expression in Escherichia coli / Z. Naturforsch 61 : 840 ~
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