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자료유형
학술저널
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한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제24권 제4호
발행연도
2014.1
수록면
534 - 544 (11page)

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The effects of microbial iron reduction and oxidation on the immobilization and mobilizationof copper were investigated in a high concentration of sulfate with synthesized Fe(III) mineralsand red earth soils rich in amorphous Fe (hydr)oxides. Batch microcosm experiments showedthat red earth soil inoculated with subsurface sediments had a faster Fe(III) bioreduction ratethan pure amorphous Fe(III) minerals and resulted in quicker immobilization of Cu in theaqueous fraction. Coinciding with the decrease of aqueous Cu, SO42- in the inoculated redearth soil decreased acutely after incubation. The shift in the microbial community compositein the inoculated soil was analyzed through denaturing gradient gel electrophoresis. Resultsrevealed the potential cooperative effect of microbial Fe(III) reduction and sulfate reductionon copper immobilization. After exposure to air for 144 h, more than 50% of the immobilizedCu was remobilized from the anaerobic matrices; aqueous sulfate increased significantly. Sequential extraction analysis demonstrated that the organic matter/sulfide-bound Cuincreased by 52% after anaerobic incubation relative to the abiotic treatment but decreased by32% after oxidation, indicating the generation and oxidation of Cu-sulfide coprecipitates in theinoculated red earth soil. These findings suggest that the immobilization of copper could beenhanced by mediating microbial Fe(III) reduction with sulfate reduction under anaerobicconditions. The findings have an important implication for bioremediation in Cucontaminatedand Fe-rich soils, especially in acid-mine-drainage-affected sites.

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