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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2008.1
- 수록면
- 419 - 427 (9page)
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The present study aimed to investigate the osteogenic potentials of differentiated osteoblast-like cells (DOCs) induced from bone marrow-derived mesenchymal stem cells (MSCs) on β-tricalcium phosphate (β-TCP) with recombinant human bone morphogenetic protein (rhBMP-2) in vitro. Osteoblast differentiation was induced in confluent cultures by adding 100 nM dexamethasone, 10 mM β-glycerophosphate, 50 mM L-ascorbic acid. The Alizarin red S staining and reverse transcriptase-polymerase chain reaction (RT-PCR) were perfomed to examine the mRNA expression of alkaline phosphatase (ALP), bone sialoprotein (BSP), osteocalcin (OCN), receptor activator for nuclear factor κB ligand (RANKL), runt-related transcription factor 2 (RUNX2), collagen-Ⅰ (COL-Ⅰ).
There were no significant differences in the osteogenic potentials of DOCs induced from MSCs on β-TCP(+/-). According to the incubation period, there were significant increasing of Alizadin red S staining in the induction 3 weeks. The mRNA expression of ALP, RUNX2, and RANKL were higher in DOCs/β-TCP(-) than DOCs/β-TCP(+). According to rhBMP-2 concentrations, the mRNA expression of BSP was significantly increased in DOCs/β-TCP(+) compared to that of DOCs/β-TCP(-) on rhBMP 10 ng/ml. Our study presented the β-TCP will have the possibility that calcium phosphate directly affect the osteoblastic differentiation of the bone marrowderived MSCs.
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참고문헌 (24)
참고문헌 신청
Aghaloo TL / 2004 / Evaluation of platelet-rich plasma in combination with anorganic bovine bone inthe rabbit cranium: a pilot study / Int J Oral Maxillofac Implants 19 : 59
Ahn SH / 2003 / Effect of recombinant human bone morphogenetic protein-4 with carriers in ratcalvarial defects / J Periodontol 74 : 787 ~ 797
Becker W / 2003 / Treatment of small defects adjacent to oral implants with various biomaterials / Periodontol 2000 33 : 26 ~ 35
Beresford JN / 1989 / Osteogenic stem cells and the stromal system of bone and marrow / Clin Orthop Rel Res 240 : 270 ~ 280
Bjerre L / 2008 / Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds / Biomaterials 29 : 2616 ~ 2627
Ahn SH / 2003 / Effect of recombinant human bone morphogenetic protein-4 with carriers in ratcalvarial defects / J Periodontol 74 : 787 ~ 797
Becker W / 2003 / Treatment of small defects adjacent to oral implants with various biomaterials / Periodontol 2000 33 : 26 ~ 35
Beresford JN / 1989 / Osteogenic stem cells and the stromal system of bone and marrow / Clin Orthop Rel Res 240 : 270 ~ 280
Bjerre L / 2008 / Flow perfusion culture of human mesenchymal stem cells on silicate-substituted tricalcium phosphate scaffolds / Biomaterials 29 : 2616 ~ 2627
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