지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 232 - 238 (7page)
이용수
이 논문의 연구 히스토리 (2)
- 이 논문의 후속연구가 궁금하신가요?
- 연관 학술논문 또는 학술발표를 통해 보다 발전된 연구결과를 확인하실 수 있습니다.
- 이 논문의 연구 히스토리 확인하기
초록· 키워드
오류제보하기
Objective The study aimed to evaluate the feasibility and reproducibility of measuring phospholipase C zeta (PLCζ) using immunostaining in human sperm and to investigate the relationship between PLCζ immunoreactivity and DNA fragmentation and oxidation in human sperm.
Methods Semen samples were obtained from participants (n=44) and processed by the conventional swim-up method. Sperm concentration, motility, normal form by strict morphology, DNA fragmentation index assessed by terminal deoxynucleotidyl transferase dUTP nick end labeling method and immunofluorescent expression for 8-hydroxy-2’-deoxyguanosine (8-OHdG) and PLCζ were assessed.
Results When duplicate PLCζ tests were performed on two sperm samples from each of the 44 participants, similar results were obtained (74.1±9.4% vs. 75.4±9.7%). Two measurements of PLCζ were found to be highly correlated with each other (r=0.759, P<0.001). Immunoreactivity of PLCζ was not associated with donor’s age, sperm concentration, motility, and the percentage of normal form as well as DNA fragmentation index. However, immunoreactivity of PLCζ showed a significant negative relationship with 8-OHdG immunoreactivity (r=-0.404, P=0.009).
Conclusion Measurement of PLCζ by immunostaining is feasible and reproducible. Lower expression of PLCζ in human sperm may be associated with higher sperm DNA oxidation status.
목차
등록된 정보가 없습니다.
참고문헌 (20)
참고문헌 신청
Aghajanpour S. / 2011 / Quantitative expression of phospholipase C zeta, as an index to assess fertilization potential of a semen sample / Hum Reprod 26 : 2950 ~ 2956
Aitken RJ. / 2010 / Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population : development of diagnostic criteria / Hum
Aitken RJ. / 2010 / On the possible origins of DNA damage in human spermatozoa / Mol Hum Reprod 16 : 3 ~ 13
Fraga CG. / 1991 / Ascorbic acid protects against endogenous oxidative DNA damage in human sperm / Proc Natl Acad Sci U S A 88 : 11003 ~ 11006
Garrido N. / 2004 / Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content
Aitken RJ. / 2010 / Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population : development of diagnostic criteria / Hum
Aitken RJ. / 2010 / On the possible origins of DNA damage in human spermatozoa / Mol Hum Reprod 16 : 3 ~ 13
Fraga CG. / 1991 / Ascorbic acid protects against endogenous oxidative DNA damage in human sperm / Proc Natl Acad Sci U S A 88 : 11003 ~ 11006
Garrido N. / 2004 / Relationship among standard semen parameters, glutathione peroxidase/glutathione reductase activity, and mRNA expression and reduced glutathione content
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Phospholipase C zeta: a hidden face of sperm for oocyte activation and early embryonic development
Obstetrics & Gynecology Science
2024 .09
-
14-3-3ζ Overexpression is Associated with Poor Prognosis in Ovarian Cancer
Yonsei Medical Journal
2018 .01
-
What should be done for men with sperm DNA fragmentation?
Clinical and experimental reproductive medicine : CERM
2018 .01
-
ICSI significantly improved the pregnancy rate of patients with a high sperm DNA fragmentation index
Clinical and experimental reproductive medicine : CERM
2017 .01
-
Does conventional freezing affect sperm DNA fragmentation?
Clinical and experimental reproductive medicine : CERM
2019 .01
-
한국형 공공정자은행의 설립과 운영
대한의사협회지
2016 .01
-
비배우자 기증 정자의 절대부족의 원인과 해결방안
대한의사협회지
2018 .01
-
Sperm DNA fragmentation and sex chromosome aneuploidy after swim-up versus density gradient centrifugation
Clinical and experimental reproductive medicine : CERM
2017 .01
-
Tobacco Use Increases Oxidative DNA Damage in Sperm - Possible Etiology of Childhood Cancer
Asian Pacific journal of cancer prevention : APJCP
2015 .01
-
Smart sperm analyzer based on cellular phone
한국실험동물학회 학술발표대회 논문집
2019 .01
-
Phospholipase C Beta 2 Protein Overexpression Is a Favorable Prognostic Indicator in Newly Diagnosed Normal Karyotype Acute Myeloid Leukemia
Annals of Laboratory Medicine
2021 .01
-
Impact of sperm DNA fragmentation on clinical in vitro fertilization outcomes
Clinical and experimental reproductive medicine : CERM
2017 .01
-
Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in IVF and ICSI
Yonsei Medical Journal
2020 .01
-
Efficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting
Clinical and experimental reproductive medicine : CERM
2016 .01
-
Comparison of mice’ sperm parameters exposed to some hazardous physical agents
Environmental Analysis Health and Toxicology
2021 .09
-
Clinical assessment of the male fertility
Obstetrics & Gynecology Science
2018 .01
-
Factors influencing sperm retrieval following testicular sperm extraction in nonobstructive azoospermia patients
Clinical and experimental reproductive medicine : CERM
2017 .01
-
The Author Reply: Effect of Sperm DNA Fragmentation on Embryo Quality in Normal Responder Women in IVF and ICSI
Yonsei Medical Journal
2020 .01
-
Oxidative Stress Induced Damage to Paternal Genome and Impact of Meditation and Yoga - Can it Reduce Incidence of Childhood Cancer?
Asian Pacific journal of cancer prevention : APJCP
2016 .01
-
Supplementation of cryoprotective extender with resveratrol decreases apoptosis index and reactive oxygen species levels in post-thaw dog sperm
Korean Journal of Veterinary Research(대한수의학회지)
2021 .12
이 논문의 저자 정보
최근 본 자료
댓글(0)
0