지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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초록· 키워드
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This study was conducted to assess genetic diversity among 16 genotypes of boxthorn (Lycium chinensis Mill.) using inter-simple sequence repeat (ISSR) markers. The 18 ISSR primers out of 100 primers showed the amplification of 101 reproducible fragments. A total of 56 DNA fragments were polymorphic with an average 3.1 polymorphic bands per primer. The polymorphic primers were divided into 16 anchored primers and 2 non-anchored primers. All of the anchored primers were di-nucleotide repeat motif, and polymorphism level was higher in the primers with poly GA and CT motif than CA and GT motif primers. Based on polymorphism, cluster analysis was conducted by the unweighted pair-group method with arithmetic average (UPGMA) methods. Sixteen boxthorn varieties and accessions were separated into 2 distinctive groups and genetic distance of cluster ranged from 0.82 to 0.97. Eighteen markers were able to distinguish every variety. Therefore, ISSR markers may be suitable for characterizing the large numbers of germplasms and fingerprinting of boxthorn varieties.
목차
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참고문헌 (19)
참고문헌 신청
Blair MW / 1999 / Inter-simple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). 98 : 780 ~ 79
Fang DQ / 1997 / Identification of closely related citrus cultivars with inter-simple sequence repeat markers 95 : 408 ~ 417
Hu J / 2003 / analysis of sweetpotato and wild relative using Inter-simple sequence repeats (ISSRs). 53 : 297 ~ 304
Kim DH / 2002 / Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats. 121 : 259 ~ 262
Lee BC / 1998 / Variation of chemical properties in collected boxthorn varieties 30 : 267 ~ 272
Fang DQ / 1997 / Identification of closely related citrus cultivars with inter-simple sequence repeat markers 95 : 408 ~ 417
Hu J / 2003 / analysis of sweetpotato and wild relative using Inter-simple sequence repeats (ISSRs). 53 : 297 ~ 304
Kim DH / 2002 / Genetic relationships of sesame germplasm collection as revealed by inter-simple sequence repeats. 121 : 259 ~ 262
Lee BC / 1998 / Variation of chemical properties in collected boxthorn varieties 30 : 267 ~ 272
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