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자료유형
학술저널
저자정보
저널정보
한국육종학회 한국육종학회지 한국육종학회지 제36권 제2호
발행연도
2004.1
수록면
76 - 80 (5page)

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초록· 키워드

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Two important loci, designated Rhg1 and Rhg4, are responsible for most resistance to soybean cyst nematode (SCN). The objective of this study was to survey single nucleotide polymorphism (SNP) and to map the SCN-resistant gene, Rhg4, on soybean genetic map. Direct sequencing with two primer sets Rhg4 gene revealed that three SNPs and seven indels were detected among six different soybean genotypes. The designed oligonucleotide complementary to the 5 region from the T(A)/C(G) SNP out of 10 SNPs detected was used in SNaPshot reactions, confirming polymorphism between PI 96188 and Jinju 1. Using a total of 90 recombinant inbred lines (RILs) derived from a cross between PI 96188?´?Jinju 1, the genetic population was mapped with 32 SSR markers, in which thirteen markers were placed into 5 linkage groups, covering 78.6 cM, and average distance between markers was 6.1 cM. Even though the genetic map was not completely saturated with SSR markers, linkage study revealed that Rhg4 was tightly linked to Satt187 on LG A2 with a distance of 6.8 cM. The location of Rhg4 on the genetic map is a good agreement with the previous report in the USDA map. From this study, it was concluded that sequence data availability of the responsible gene for the SCN resistance and sequence polymorphism between two parents map the gene successfully without phenotypic evaluation. In addition, SNP marker for SCN resistance map may be used in a marker-assisted selection for development of SCN-resistant soybean varieties in a breeding program.

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