지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
The carotenoid-biofortified (PAC) rice was developed and confirmed to synthesis carotenoids (zeaxanthin, α-carotene and β-carotene) in rice endosperm. For biosafety assessment of PAC rice, the stability of the insert genes in carotenoidbiofortified rice at genomic and protein expression level through different generations (T3~T6) was studied. Southern blot analysis confirmed that the multi-generation stability of the genes : PAC gene (CrtI and Psy), promoters (35S and Glb), terminators (NOS and PinII), selectable marker (bar) and MAR gene. It was also confirmed that the backbone DNA containing antibacterial gene (aadA) was not present in PAC rice. Immuno-strip assay (PAT) showed that the bar gene was stably expressed through generations. HPLC analysis confirmed that carotenoids were consistently detected through T3~T6 generations. These results will be filed to biosafety assessment document of PAC rice.
목차
등록된 정보가 없습니다.
참고문헌 (11)
참고문헌 신청
Datta K / 2003 / Bioengineered“golden” indica rice cultivars with β-carotene metabolism in the endosperm with hygromycin and mannose selection systems / Plant Biotech J 1
De Vries J / 2004 / Microbial horizontal gene transfer and the DNA release from transgenic crop plants / Plant and Soil 266 : 91 ~ 104
Ha SH / 201010 / Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm / PLANT BIOTECHNOLOGY JOURNAL 8 (
Hoa T / 2003 / Golden Indica and Japonica rice lines amenable to deregulation / Plant Physiol 133 : 161 ~ 169
James C / 2011 / The global status of commercialized biotech/GM crops: 2011
De Vries J / 2004 / Microbial horizontal gene transfer and the DNA release from transgenic crop plants / Plant and Soil 266 : 91 ~ 104
Ha SH / 201010 / Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm / PLANT BIOTECHNOLOGY JOURNAL 8 (
Hoa T / 2003 / Golden Indica and Japonica rice lines amenable to deregulation / Plant Physiol 133 : 161 ~ 169
James C / 2011 / The global status of commercialized biotech/GM crops: 2011
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