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초록· 키워드
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The β-carotene biofortified transgenic soybean was developed recently through Agrobacterium-mediated transformation using the recombinant PAC (Phytoene synthase-2A-Carotene desaturase) gene in Korean soybean (Glycine max L. cv. Kwangan).
GM crops prior to use as food or release into the environment required risk assessments to environment and human health in Korea. Generally, transgenic plants containing a copy of T-DNA were used for stable expression of desirable trait gene in risk assessments. Also, information about integration site of T-DNA can be used to test the hypothesis that the inserted DNA does not trigger production of unintended transgenic proteins, or disrupt plant genes, which may cause the transgenic crop to be harmful. As these reasons, we selected four transgenic soybean lines expressing carotenoid biosynthesis genes with a copy of T-DNA by using Southern blot analysis, and analyzed the integration sites of their T-DNA by using flanking sequence analysis.
The results showed that, T-DNA of three transgenic soybean lines (7-1-1-1, 9-1-2, 10-10-1) was inserted within intergenic region of the soybean chromosome, while T-DNA of a transgenic soybean line (10-19-1) located exon region of chromosome 13. This data of integration site and flanking sequences is useful for the biosafety assessment and for the identification of the β-carotene biofortified transgenic soybean.
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참고문헌 (16)
참고문헌 신청
Bugert P / 2001 / Improved PCRwalking for large-scale isolation of plant T-DNA borders / Bio. Techniques 30 : 496 ~ 504
De Buck S / 2004 / Single-copy T-DNAs integrated at different positions in the Arabidopsis genome display uniform and comparable β-glucuronidase accumulation levels / Cell M
Doyle JJ / 1987 / A rapid DNA isolation procedure for small quantities of fresh leaf tissue / Phytochemical Bulletin 19 : 11 ~ 15
Gheysen G / 1991 / Illegitimate recombination in plants: a model for T-DNA integration / Genes Dev 5 : 287 ~ 297
Ha SH / 2007 / A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: Deletion of the capsanthin-capsorubin synthase gene is
De Buck S / 2004 / Single-copy T-DNAs integrated at different positions in the Arabidopsis genome display uniform and comparable β-glucuronidase accumulation levels / Cell M
Doyle JJ / 1987 / A rapid DNA isolation procedure for small quantities of fresh leaf tissue / Phytochemical Bulletin 19 : 11 ~ 15
Gheysen G / 1991 / Illegitimate recombination in plants: a model for T-DNA integration / Genes Dev 5 : 287 ~ 297
Ha SH / 2007 / A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: Deletion of the capsanthin-capsorubin synthase gene is
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