지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
Beta-carotene producing transformants were produced in the background of ‘Nagdongbyeo’, a Japonica rice cultivar.
Introgression of the carotenoid locus in the transformant, PAC4-2 into the elite cultivar ‘Ilpumbyeo’ was started. To initiate a backcrossing program, we surveyed 220 SSR markers and found that 38% of them were polymorphic between ‘Ilpumbyeo’ as a recurrent parent and the PAC4-2 as a recipient parent.
The selection strategy comprising foreground and background selection was employed. First, foreground selection was practiced in BC_1, BC_2, and BC_3 generations using the transgene specific PCR-based marker in addition to visual scoring of the seed color.
Marker-based background selection combined with phenotypic selection was employed from BC_3F_2 to BC_3F_4 generations. Blast search indicated that the transgene PAC4-2 was located between SSR markers, RM6 and RM482. 240 BC_3F_3 and 63 BC_3F_4 lines were evaluated for four agronomic traits including days to heading. Most of the lines were similar to Ilpumbyeo in agronomic traits evaluated. The percentage of PAC4-2 genome ranged from 4% to 21% with a mean of 12.5%, which was higher than the expected for an unselected BC_3 backcross population. This could be explained by the fact that two genes for beta-carotene and the stripe virus resistance were targeted in this study. We selected 10 representative BC_3F_5 lines from 63 BC_3F_4 lines based on agronomic traits and carotenoids content. The selection strategy would be appropriate for the introgression of beta-carotene gene in a breeding program.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Ashikari M / 2005 / Cytokinin oxidase regulates rice grain production / Science 309 : 741 ~ 745
Causse MA / 1994 / Saturated molecular map of the rice genome based on an interspecific backcross population / Genetics 138 : 1251 ~ 1274
Collard BCY / 2008 / Marker-assisted selection: an approach for precision plant breeding in the 21st centuty / Phil Trans Royal Soc B Rev 363 : 557 ~ 572
Ha SH / 2007 / A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: deletion of the capsanthin-capsorubin synthase gene is
Ha SH / 2010 / Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm / PLANT BIOTECHNOLOGY JOURNAL 8 (8)
Causse MA / 1994 / Saturated molecular map of the rice genome based on an interspecific backcross population / Genetics 138 : 1251 ~ 1274
Collard BCY / 2008 / Marker-assisted selection: an approach for precision plant breeding in the 21st centuty / Phil Trans Royal Soc B Rev 363 : 557 ~ 572
Ha SH / 2007 / A comparison of the carotenoid accumulation in Capsicum varieties that show different ripening colours: deletion of the capsanthin-capsorubin synthase gene is
Ha SH / 2010 / Application of two bicistronic systems involving 2A and IRES sequences to the biosynthesis of carotenoids in rice endosperm / PLANT BIOTECHNOLOGY JOURNAL 8 (8)
이 논문의 저자 정보
최근 본 자료
댓글(0)
0