지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Genetic diversity among 28 Cymbidium varieties was evaluated by using a sequence-related amplified polymorphism (SRAP) marker system. The SRAP marker which was based on the open reading frames (ORFs) regions was developed primarily for Brassica species, but has been applied to various crops. A total of 30 SRAP primer combinations were initially screened.
Twenty-eight SRAP primer combinations showed high polymorphism among the 28 Cymbidium varieties, which were consisted of breeding varieties and their parents in National Institute of Horticultural & Herbal Science (NIHHS). The amplified DNA fragments were separated by denaturing acrylamide gels and detected silver staining method. One hundred ninety six polymorphic bands (7 per primer) were generated and ranged from 0.3 to 1.0 kb in size. Polymorphic fragments were scored for calculating simple matching coefficient of genetic similarity and cluster analysis with multi-variate statistical package (MVSP) 3.1. The mean genetic similarity coefficient value was 0.588. The results showed that the correlation between F_1 varieties and their parents was high. These studied SRAP markers will be useful tools for genotype identification, germplasm conservation, genetic relationships in Cymbidium.
목차
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참고문헌 (17)
참고문헌 신청
Chang YK / 2009 / Analysis of genetic variability among Phalaenopsis species and hybrids using amplified fragment length polymorphism / J. Amer. Soc. Hort. Sci 134 (1) : 58 ~
Choi SH / 2006 / Genetic diversity and phylogenetic relationships among and within species of oriental cymbidiums based on RAPID analysis / SCIENTIA HORTICULTURAE 108 : 79 ~
Chung SY / 2006 / Genetic relationship and differentiation of Paphiopedilum and Phragmepedium based on RAPD analysis / SCIENTIA HORTICULTURAE 109 : 153 ~ 159
Ferriol M / 2003 / Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers / Theor Appl Genet 107 : 271 ~ 282
Huang Y / 2010 / Analysis of diversity and relationships and among Chinese orchid cultivars using EST-SSR markers / Biochem. Syst. Ecol 38 : 93 ~ 102
Choi SH / 2006 / Genetic diversity and phylogenetic relationships among and within species of oriental cymbidiums based on RAPID analysis / SCIENTIA HORTICULTURAE 108 : 79 ~
Chung SY / 2006 / Genetic relationship and differentiation of Paphiopedilum and Phragmepedium based on RAPD analysis / SCIENTIA HORTICULTURAE 109 : 153 ~ 159
Ferriol M / 2003 / Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers / Theor Appl Genet 107 : 271 ~ 282
Huang Y / 2010 / Analysis of diversity and relationships and among Chinese orchid cultivars using EST-SSR markers / Biochem. Syst. Ecol 38 : 93 ~ 102
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