지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
오류제보하기
We used suppression subtractive hybridization (SSH) combined with mirror orientation selection (MOS) method to screen differentially expressed genes from red-fleshed kiwifruit ‘Hongyang’. As a result, the 288 clones were obtained by subcloning PCR product and 192 clones that showed positive clones on colony PCR analysis were selected. All the positive clones were sequenced. After comparisons with the NCBI/Genbank database using the BLAST search revealed that 30 clones showed sequence similarity to genes from other organisms; 10 clones showed significant sequence similarity to known genes.
Among these clones, 3 clones (AcF21, AcF42 and AcF106) had sequence homology to 1-aminicyclopropane-carboxylic acid (ACC)-oxidase (ACO) that known to be related to fruit ripening. The expression patterns of differentially expressed genes were further investigated to validate the SSH data by reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qReal-time PCR) analysis. All the data from qReal-time PCR analysis coincide with the results obtained from RT-PCR analysis. Three clones were expressed at higher levels in ‘Hongyang’ than ‘Hayward’. AcF21 was highly expressed in the other genes at 120 days after full bloom (DAFB) and 160 DAFB of ‘Hongyang’.
목차
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참고문헌 (24)
참고문헌 신청
Anjanasree KN / 2005 / Differential expression of tomato ACC oxidase gene family in relation to fruit ripening / Curr Sci 89 : 1394 ~ 1399
Atkinson RG / 2011 / Dissecting the role of climacteric ethylene in kiwifruit (Actinidia chinensis) ripening using an 1-aminicyclopropane- 1-carboxylic acid oxidase knockdown
Ban Y / 2007 / Suppression subtractive hybridization identifies genes induced in response to UV-B irradiation in apple skin: isolation of a putative UDP-glucose 4-epimerase /
Barry CS / 1996 / Differential expression of the 1- aminocyclopropane-1-carboxylate oxidase gene family of tomato / The Plant J 9 : 525 ~ 535
Chang S / 1993 / A simple and efficient method for isolating RNA from pine trees / Plant Mol Biol Rep 11 : 113 ~ 116
Atkinson RG / 2011 / Dissecting the role of climacteric ethylene in kiwifruit (Actinidia chinensis) ripening using an 1-aminicyclopropane- 1-carboxylic acid oxidase knockdown
Ban Y / 2007 / Suppression subtractive hybridization identifies genes induced in response to UV-B irradiation in apple skin: isolation of a putative UDP-glucose 4-epimerase /
Barry CS / 1996 / Differential expression of the 1- aminocyclopropane-1-carboxylate oxidase gene family of tomato / The Plant J 9 : 525 ~ 535
Chang S / 1993 / A simple and efficient method for isolating RNA from pine trees / Plant Mol Biol Rep 11 : 113 ~ 116
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