지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40g/L sucrose and 2,4-D, NAA, 2,4,5-T and dicamba 24 h after pollination, and then incubated until embryo rescue. Fourteen to sixteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/L sucrose and 1 mg/L NAA. The type of plant growth regulators was found to be most significant in production of haploid plants.
The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of seeded florets was significantly affected by 100 mg/L 2,4-D, 150 mg/L 2,4,5-T and 50 mg/L dicamba. The percentage of embryos formed was significantly increased by treatment with 2,4-D and 2,4,5-T at 100 mg/L, and dicamba at 50 mg/L, but the treatments with 150 mg/L 2,4-D inhibited embryo development and plant regeneration. The optimum application time of plant growth regulators was 24 hrs after pollination.
목차
등록된 정보가 없습니다.
참고문헌 (27)
참고문헌 신청
Ahmad J / 2004 / Improvements in wheat x maize crossing system of doubled haploid production in bread wheat / 박사 / University of Agriculture
Almouslem AB / 1998 / Haploid durum wheat production via hybridization with maize / Crop Sci 38 : 1080 ~ 1087
Beom-Young Son / 2006 / A New Corn Hybrid Cultivar, “Gangdaok” for Silage / 한국육종학회지 38 (2) : 147 ~ 148
Bernier G / 1981 / The physiology of flowering. Vol 2 / CRC Press : 1 ~ 231
Brazauskas G / 2005 / Improved approaches in wheat × maize crossing for wheat doubled haploid production / Biologija. Nr 4 : 15 ~ 18
Almouslem AB / 1998 / Haploid durum wheat production via hybridization with maize / Crop Sci 38 : 1080 ~ 1087
Beom-Young Son / 2006 / A New Corn Hybrid Cultivar, “Gangdaok” for Silage / 한국육종학회지 38 (2) : 147 ~ 148
Bernier G / 1981 / The physiology of flowering. Vol 2 / CRC Press : 1 ~ 231
Brazauskas G / 2005 / Improved approaches in wheat × maize crossing for wheat doubled haploid production / Biologija. Nr 4 : 15 ~ 18
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