지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제29권 제2호
- 발행연도
- 2014.1
- 수록면
- 185 - 192 (8page)
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Recombinant proteins can be generated quickly and easily in large amounts and at low-costin silkworm larvae by using Bombyx mori nuclear polyhedrosis virus (BmNPV). We searchedfor high-permissive silkworm strains that have high production levels of heterologous proteinsand are thus suitable for use as biofactories. In this study, we performed the analysis usinga BmNPV vector expressing luciferase as a marker, and we confirmed protein expressionby evaluating luciferase activity, determined by western blotting and luciferase ELISA, andconfirmed transcription expression by semi- and quantitative real time PCR. For the selectionof host silkworm strains, we first chose 52 domestic BmNPV sensitive strains and thenidentified 10 high-permissive and 5 low-permissive strains. In addition, to determine whichhybrid of the high-permissive strains would show heterosis, nine strains derived through threewaycrossing were tested for luciferase activity by western blotting, and luciferase ELISA.
We found a correlation between luciferase activity and luciferase protein expression, but nottranscription. There was no noticeable difference in protein expression levels between Jam313as the high-permissive control strain and the three-way hybrid strains; however, the three-waycross strains showed lower luciferase activity compared with Jam313. In this study, luciferaseprotein production in the larvae of 52 domestic silkworm strains was elucidated using BmNPV.
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참고문헌 (24)
참고문헌 신청
Christensen T / 1988 / High level expression of recombinant genes in Aspergillus oryzae / Bio/Technology 6 : 1419 ~ 1422
Cregg JM / 1993 / Recent advances in the expression of foreign genes in Pichia pastoris / Bio/Technology 11 : 905 ~ 910
Dartar RV / 1993 / Process economics of animal cell and bacterial fermentations: a case study analysis of tissue plasminogen activator / Bio/Technology 11 : 349 ~ 357
Demain AL / 2009 / Production of recombinant proteins by microbes and higher organisms / Biotechnology Advances 27 : 297 ~ 306
Goodrick JC / 2001 / High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system / Biotech
Cregg JM / 1993 / Recent advances in the expression of foreign genes in Pichia pastoris / Bio/Technology 11 : 905 ~ 910
Dartar RV / 1993 / Process economics of animal cell and bacterial fermentations: a case study analysis of tissue plasminogen activator / Bio/Technology 11 : 349 ~ 357
Demain AL / 2009 / Production of recombinant proteins by microbes and higher organisms / Biotechnology Advances 27 : 297 ~ 306
Goodrick JC / 2001 / High-level expression and stabilization of recombinant human chitinase produced in a continuous constitutive Pichia pastoris expression system / Biotech
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