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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국잠사학회 International Journal of Industrial Entomology International Journal of Industrial Entomology 제20권 제2호
- 발행연도
- 2010.1
- 수록면
- 107 - 114 (8page)
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초록· 키워드
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For stable germline transformation, the promoter of Bombyx mori cytoplasmic actin gene (BmA3) has been used for ubiquitous expression of transgenes. So far,no strong promoter is available for ubiquitous expression in B. mori, excluding BmA3 promoter. To identify more powerful promoter than previously reported BmA3 promoter, we isolated 9 clones that show stronger signal compared to BmA3 by a dot blot hybridization.
Among these 9 clones, we focused on one clone which has high amino acid homology (85%) with hypothetical protein 32 gene of Lonomia obliqua. This clone, named bHp32 (B. mori hypothetical protein 32)was ubiquitously expressed in all tissues and developmental stage of fifth instar B. mori larvae. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-1,200/+220) in the 5’-flanking region of bHp32 gene, which has 42-fold more intensive promoter activity than BmA3 promoter. Moreover, the bHp32 promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore,we suggest that bHp32 promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.
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참고문헌 (21)
참고문헌 신청
Ailor E / 1999 / Modifying secretion and posttranslational processing in insect cells / Curr Opin Biotechnol 10 : 142 ~ 145
Goo TW / 2002 / Molecular characterization of a Bombyx mori protein disulfide isomerase (bPDI) / Cell Stress Chaperones 7 : 118 ~ 125
Handler AM / 1998 / The Lepidopteran transposon vector, piggyBac, mediates germline transformation in the Mediterranean fruit fly / Proc Natl Acad Sci USA : 7520 ~ 7525
Hoog C / 1991 / Isolation of a large number of novel mammalian genes by a differential cDNA library screening strategy / Nucleic Acids Res 19 : 6123 ~ 6127
Horn C / 2002 / Fluorescent transformation markers for insect transgenesis / Insect Biochem Mol Biol 32 : 1221 ~ 1235
Goo TW / 2002 / Molecular characterization of a Bombyx mori protein disulfide isomerase (bPDI) / Cell Stress Chaperones 7 : 118 ~ 125
Handler AM / 1998 / The Lepidopteran transposon vector, piggyBac, mediates germline transformation in the Mediterranean fruit fly / Proc Natl Acad Sci USA : 7520 ~ 7525
Hoog C / 1991 / Isolation of a large number of novel mammalian genes by a differential cDNA library screening strategy / Nucleic Acids Res 19 : 6123 ~ 6127
Horn C / 2002 / Fluorescent transformation markers for insect transgenesis / Insect Biochem Mol Biol 32 : 1221 ~ 1235
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