지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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초록· 키워드
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We have evaluated the effects of experimental factors on transmitted electron beam intensities for quantitative analysis
in electron tomography. For the correct application of Beer’s law in electron tomography, the transmitted beam intensity
should reflect the net effect of mass properties on beam path. So, the any other effects of the objective aperture and the
specimen holder on beam path should be removed. The cut-off effects of objective aperture were examined using Quantifoil
holey carbon film and a transmission electron microscope operated at 120 kV. The transmitted beam intensities with
30 μm objective aperture dropped about 16.7% compared to electron beam intensities without the objective aperture. Also,
the additional losses of about 14.2% at high tilt angles were occurred by cut-off effects of the objective apertures. For the
precise quantitative analysis in electron tomography, the effect of the objective aperture on transmitted electron beam
intensities should be considered. It is desirable that 2-D tilt series images are obtained without the objective aperture for
correct application of Bee’s law.
목차
등록된 정보가 없습니다.
참고문헌 (10)
참고문헌 신청
Danev R / 2008 / Single particle analysis based on zernike phase contrast microscopy / J Struct Biol 161 : 211 ~ 218
DeRosier DJ / 1968 / Reconstruction of three dimensional struc-tures from electron micrographs / Nature 217 : 130 ~ 134
Friedricha H / 2005 / Comparison of intensity distributions in tomograms from BFTEM, ADFSTEM, HAADFSTEM, and calculated tilt series / Ultramicroscopy 106 : 18 ~ 27
Hart RG / 1968 / Electron microscopy of unstained biological material: the polytropic montage / Science 159 : 1464 ~ 1467
Midgley PA / 2003 / 3D electron microscopy in the physical sciences: the development of Z-contrast and EFTEM tomography / Ultramicroscopy 96 : 413 ~ 431
DeRosier DJ / 1968 / Reconstruction of three dimensional struc-tures from electron micrographs / Nature 217 : 130 ~ 134
Friedricha H / 2005 / Comparison of intensity distributions in tomograms from BFTEM, ADFSTEM, HAADFSTEM, and calculated tilt series / Ultramicroscopy 106 : 18 ~ 27
Hart RG / 1968 / Electron microscopy of unstained biological material: the polytropic montage / Science 159 : 1464 ~ 1467
Midgley PA / 2003 / 3D electron microscopy in the physical sciences: the development of Z-contrast and EFTEM tomography / Ultramicroscopy 96 : 413 ~ 431
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