지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Induction of neurogenesis can occur in the hippocampus in response to various pathological conditions, such as Alzheimer’s disease. The aim of this study was to investigate the changes that occur in endogenous neural stem cells in response to amyloid beta (Aβ)25-35-induced neuronal cell damage in organotypic hippocampal slice cultures. Cresyl violet staining and Fluoro-Jade B staining were used to detect neuronal cell damage and changes of mossy fiber terminals were observed by Timm’s staining. The immunofl uorescence staining was used to detect the newly generated cells in the subgranular zone (SGZ) of the dentate gyrus with specific marker, 5-bromo-2′-deoxyuridine (BrdU), Ki-67, Nestin, and doublecortin (DCX). In compared to control slices, neuronal cell damage was observed and the mossy fi bers were expanded to CA3 area by treatment with Aβ25-35. Ki-67/Nestin- and BrdU/DCX-positive cells were detected in the SGZ. In conclusion, these results demonstrate that Aβ-induced neuronal damage results in an increase in endogenous neural stem cells in rat hippocampal slice cultures not only for gliosis but also for neurogenesis.
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참고문헌 (22)
참고문헌 신청
Bjorklund N L / 2012 / Absence of amyloid beta oligomers at the postsynapse and regulated synaptic Zn2+ in cognitively intact aged individuals with Alzheimer’s disease neuro
Bunk E C / 2010 / NMDAinduced injury of mouse organotypic hippocampal slice cultures triggers delayed neuroblast proliferation in the dentate gyrus: an in vitro model for the
Bush A I / 1994 / Rapid induction of Alzheimer A beta amyloid formation by zinc / Science 265 : 1464 ~ 1467
Chechneva O / 2005 / Identification and characterization of two neurogenic zones in interface organotypic hippocampal slice cultures / Neuroscience 136 : 343 ~ 355
Chuang T T / 2010 / Neurogenesis in mouse models of Alzheimer’s disease / BBA 1802 : 872 ~ 880
Bunk E C / 2010 / NMDAinduced injury of mouse organotypic hippocampal slice cultures triggers delayed neuroblast proliferation in the dentate gyrus: an in vitro model for the
Bush A I / 1994 / Rapid induction of Alzheimer A beta amyloid formation by zinc / Science 265 : 1464 ~ 1467
Chechneva O / 2005 / Identification and characterization of two neurogenic zones in interface organotypic hippocampal slice cultures / Neuroscience 136 : 343 ~ 355
Chuang T T / 2010 / Neurogenesis in mouse models of Alzheimer’s disease / BBA 1802 : 872 ~ 880
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