지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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For TEM study of biological samples or polymers that are contained in organic structure, it is often required that the sample is prepared by using ultramicrotome and stained with proper agents to increase the contrast of organic structure. In this study,we investigated an efficient TEM sample preparation method for ductile heterogeneity material by using ultramicrotomy. Cryo-ultramicrotomy is a suitable method that is capable of rendering sample hardness for various ductile materials. However, it has several factors to consider, such as experimental cost, working time and finding the optimal staining conditions. To satisfy these considerations,we prepared TEM sample by using ultramicrotome without cryofunction,and secured the sample hardness by applying the staining process prior to ultrathin sectioning. The cross-linked polyethylene structure in the sample was stained with the 2% RuO4 solution in a sealed test tube for 24 hours at 4C. After the sample staining, ultrathin sections of sample were prepared using ultramicrotome. As a result, it was revealed that the difficulties associated with staining of ultrathin sections prepared by low-temperature conditions were improved. In addition, appropriate staining depth of sample could be selected for sectioning process. The quality of TEM sample obtained by using this method was better than that of cryo-ultramicroscopy.
Finally, it is expected that our method could be effectively applied in TEM sample preparation for a variety of nano-bio convergence materials.
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참고문헌 (11)
참고문헌 신청
Ayache J / 2009 / Sample preparation handbook for transmission electron microscopy / Springer : 201 ~ 228
Bozzola JJ / 1992 / Electron Microscopy / Jones & Bartlett Learning : 72 ~ 82
Chou TM / 2002 / Nanoscale artifacts in RuO4-stained poly(styrene) / Polymer 43 (7) : 2085 ~ 2088
Jeong KU / 2009 / Polymer structure analysis utilizing TEM and SAED / Polymer Science and Technology 20 (2) : 180 ~ 185
Kato K / 1967 / The osmium tetroxide procedure for light and electron microscopy of ABS plastics / Polymer Engineering and Science 7 (1) : 38 ~ 39
Bozzola JJ / 1992 / Electron Microscopy / Jones & Bartlett Learning : 72 ~ 82
Chou TM / 2002 / Nanoscale artifacts in RuO4-stained poly(styrene) / Polymer 43 (7) : 2085 ~ 2088
Jeong KU / 2009 / Polymer structure analysis utilizing TEM and SAED / Polymer Science and Technology 20 (2) : 180 ~ 185
Kato K / 1967 / The osmium tetroxide procedure for light and electron microscopy of ABS plastics / Polymer Engineering and Science 7 (1) : 38 ~ 39
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