지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
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Background: Culture based technique, a traditional method for extraction of DNA from a cultured colony, was complex in culture conditions and was associated with a lower chance of successful culture.
Recently, non-culture based technique, which skipped the culture process and directly extracted fungal DNA and differentiated Malassezia species, has been introduced.
Objective: Using 26S rDNA PCR-RFLP, the authors identified Malassezia yeasts and compared the yield of Malassezia DNA by the traditional culture based technique and the non-culture based technique via Op-site adhesive tape.
Methods: DNA of Malassezia yeasts were extracted using the culture based technique and the non-culture based technique from normal adults. Comparison was performed in order to clarify the differences between these two techniques.
Results: Use of the culture based technique resulted in a culture rate of 57.8% (78 out of 135 samples).
On the other hand, using the non-culture based technique, fungal species were identified from all 135samples. Using both techniques, M. globosa was the most identified species. The identification rate of the non-culture based technique was 100%; however, 7 repeats of PCR were required to reach 100%identification. Among samples from five body sites, those from the thigh required 5.5 repeats of PCR.
Conclusion: The non-culture based technique was better than the culture based technique. However,due to the low amount of DNA extracts from the body sites with low habitation of Malassezia yeasts,repeated PCR was required for differentiation of Malassezia species.
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참고문헌 (25)
참고문헌 신청
Ahn KJ / 1997 / Malassezia species cultured from the lesions of pityriasis versicolor / Korean J Dermatol 35 : 736 ~ 743
Ahn KJ / 1998 / Taxonomy of the genus Malassezia / Kor J Med Mycol 3 : 81 ~ 88
Baillon EH / 1889 / Traite de botanique medicale cryptogamique, suivi dutableau de droguier de la Faculte de medecine de Paris / Doin, Paris
Cha HG / 1993 / The study of incidence and distribution of Pityrospoum on clinically normal skin / Korean J Dermatol 31 : 548 ~ 558
Faergemann J / 1980 / Age incidence of Pityrosporum orbiculare on human skin / Acta Derm Venereol 60 : 531 ~ 533
Ahn KJ / 1998 / Taxonomy of the genus Malassezia / Kor J Med Mycol 3 : 81 ~ 88
Baillon EH / 1889 / Traite de botanique medicale cryptogamique, suivi dutableau de droguier de la Faculte de medecine de Paris / Doin, Paris
Cha HG / 1993 / The study of incidence and distribution of Pityrospoum on clinically normal skin / Korean J Dermatol 31 : 548 ~ 558
Faergemann J / 1980 / Age incidence of Pityrosporum orbiculare on human skin / Acta Derm Venereol 60 : 531 ~ 533
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