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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 충북대학교 동물의학연구소 Journal of Biomedical and Translational Research Journal of Biomedical and Translational Research 제20권 제3호
- 발행연도
- 2019.1
- 수록면
- 53 - 57 (5page)
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초록· 키워드
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The purpose of this study was to investigate diethylnitrosamine (DEN)-induced liver damage in zebrafish. Zebrafish larvae were divided into five groups after seventy-two hours fertilization: group 1 (G1) as control, group 2 (G2) as probe control, groups 3, 4, and 5 (G3, G4, and G5) as DEN treated at doses of 25, 50, and 100 μg/mL, respectively. At twenty-two hours after DEN treatment, groups 2, 3, 4, and 5 were treated with ApoFlamma H 675 at a dose of 100 μM/zebrafish. They were examined by fluorescence stereomicroscope at twenty-four hours after DEN treatment. After fixation, the zebrafish were processed, embedded, sectioned and stained with hematoxylin and eosin (HE) and terminal deoxynucleotide transferase dUTP nick end labeling (TUNEL) staining. Fluorescence intensity of the livers of G3, G4, and G5 was significantly increased compared with those of G1 (p<0.01). Furthermore, fluorescence intensity of the livers of G3 and G5 was significantly increased compared with those of G2 (p<0.05 and p<0.01). HE staining showed cell deaths in the livers of DEN-treated zebrafish and TUNEL staining confirmed cell death in the same location. Taken together, in vivo fluorescence bioimaging detected cell death in the liver of DEN-treated zebrafish. This outcome was confirmed with histopathological examination. The results of this study provide confidence for using zebrafish as a liver carcinogenesis model.
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참고문헌 (23)
참고문헌 신청
Rekha RD / 2008 / Thioacetamide accelerates steatohepatitis, cirrhosis and HCC by expressing HCV core protein in transgenic zebrafish Danio rerio / Toxicology 243:11~22
Menke AL / 2011 / Normal anatomy and histology of the adult zebrafish / Toxicol Pathol 39:759~775
Wiley DS / 2017 / Chemical screening in zebrafish for novel biological and therapeutic discovery / Methods Cell Biol 138:651~679
Spitsbergen JM / 2000 / Neoplasia in zebrafish (Danio rerio)treated with 7,12-dimethylbenz[a]anthracene by two exposure routes at different developmental stages / Toxicol Pathol 28:705~715
Spitsbergen JM / 2000 / Neoplasia in zebrafish(Danio rerio)treated with N-methyl-N'-nitro-N-nitrosoguanidine by three exposure routes at different developmental stages / Toxicol Pathol 28:716~725
Menke AL / 2011 / Normal anatomy and histology of the adult zebrafish / Toxicol Pathol 39:759~775
Wiley DS / 2017 / Chemical screening in zebrafish for novel biological and therapeutic discovery / Methods Cell Biol 138:651~679
Spitsbergen JM / 2000 / Neoplasia in zebrafish (Danio rerio)treated with 7,12-dimethylbenz[a]anthracene by two exposure routes at different developmental stages / Toxicol Pathol 28:705~715
Spitsbergen JM / 2000 / Neoplasia in zebrafish(Danio rerio)treated with N-methyl-N'-nitro-N-nitrosoguanidine by three exposure routes at different developmental stages / Toxicol Pathol 28:716~725
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