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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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BALB/c mice were vaccinated with Brucella (B.) abortus recombinant protein L27 (50S ribosomal protein L27) cloned into a pMal vector system. L27 was induced, purified and injected intraperitoneally (IP). Mice were vaccinated on 0-, 15- and 35-day. Serum cytokines were evaluated on 36- and 49-day from first vaccination. Mice were intraperitoneally infected with 5×104 CFU of virulent B. abortus 544 on day-50 and sacrificed after two weeks from infection. Bacterial burden from the spleen was quantified and showed a 0.7- and 0.9-log reduction in vaccinated mice in comparison to PBS and MBP (maltose binding protein) groups respectively. Cytokines in the serum demonstrated increased interferon-gamma (IFN-γ) and other pro-inflammatory cytokines such as macrophage chemoattractant protein-1 (MCP-1) and interleukin 6 (IL-6). On the other hand, interleukin 10 (IL-10) was attenuated in the sera of vaccinated mice. This cytokine profile is indicative of a cell-mediated type of immune response which is favorable for the eradication of intracellular infections. The current study showed the potential of another B. abortus ribosomal protein in inducing protective immunity against B. abortus infection.
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참고문헌 (18)
참고문헌 신청
Barquero-Calvo E / 2007 / Brucella abortus uses a stealthy strategy to avoid activation of the innate immune system during the onset of infection / PloS One 2:e631
Cassataro J / 2007 / A DNA vaccine coding for the chimera BLSOmp31 induced a better degree of protection against B. ovis and a similar degree of protection against B. melitensis than Rev.1vaccination 25:5958~5967
Dorneles E / 2015 / Immune response triggered by Brucella abortus following infection or vaccination / Vaccine 33:3659~3666
Dubray G / 1980 / Isolation of three Brucella abortus cell wall antigens protective in murine experimental brucellosis / Ann Rech Vet 11:367~373
Fernandes D / 1995 / Interleukin-10 downregulates protective immunity to Brucella abortus / Infect Immun 63:1130~1133
Cassataro J / 2007 / A DNA vaccine coding for the chimera BLSOmp31 induced a better degree of protection against B. ovis and a similar degree of protection against B. melitensis than Rev.1vaccination 25:5958~5967
Dorneles E / 2015 / Immune response triggered by Brucella abortus following infection or vaccination / Vaccine 33:3659~3666
Dubray G / 1980 / Isolation of three Brucella abortus cell wall antigens protective in murine experimental brucellosis / Ann Rech Vet 11:367~373
Fernandes D / 1995 / Interleukin-10 downregulates protective immunity to Brucella abortus / Infect Immun 63:1130~1133
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