지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제1호
- 발행연도
- 2019.1
- 수록면
- 114 - 126 (13page)
이용수
초록· 키워드
오류제보하기
This paper introduces three ways to determine host-guest complexation of cucurbit[7]uril (CB[7]) with homocysteine (Hcy). After preincubating Hcy and cysteine (Cys) with CB[7], Ellman’s reagent (DTNB) was used to detect Hcy and Cys. Only Cys reacted with DTNB and Hcy gave a retarded color change. This suggests that the -SH group of Hcy is buried inside CB[7]. Human cystathionine γ-lyase (hCGL) decreased the level of Hcy degradation after preincubating Hcy and CB[7]. These results suggest that the amount of free Hcy available was decreased by the formation of a Hcy-CB[7] complex. The immunological signal of anti-Hcy monoclonal antibody was decreased significantly by preincubating CB[7] with Hcy. The ELISA results also show that ethanethiol group (-CH2CH2SH) of Hcy, which is an epitope of anti-Hcy monoclonal antibody, was blocked by the cavity in CB[7]. Overall, CB[7] can act as a host by binding selectively with Hcy, but not Cys. The calculated half-complexation formation concentration of CB[7] was 58.2 nmol using Ellman’s protocol, 97.9 nmol using hCGL assay and 87.7 nmol using monoclonal antibody. The differing binding abilities of Hcy and Cys towards the CB[7] host may offer a simple and useful method for determining the Hcy concentration in plasma or serum.
목차
등록된 정보가 없습니다.
참고문헌 (34)
참고문헌 신청
Clarke R / 1998 / Vitamin B12, and serum total homocysteine levels in confirmed Alzheimer disease / Arch. Neurol 55:1449~1455
Nygård O / 1995 / Total plasma homocysteine and cardiovascular risk profile : the Hordaland homocysteine study / JAMA 274:1526~1533
Ueland PM / 2000 / The controversy over homocysteine and cardiovascular risk / Am. J. Clin. Nutr 72:324~332
Özkan Y / 2002 / Plasma total homocysteine and cysteine levels as cardiovascular risk factors in coronary heart disease / Int. J. Cardiol 82:269~277
Finkelstein J / 1998 / The metabolism of homocysteine: pathways and regulation / Eur. J. Pediatr 157:S40~S44
Nygård O / 1995 / Total plasma homocysteine and cardiovascular risk profile : the Hordaland homocysteine study / JAMA 274:1526~1533
Ueland PM / 2000 / The controversy over homocysteine and cardiovascular risk / Am. J. Clin. Nutr 72:324~332
Özkan Y / 2002 / Plasma total homocysteine and cysteine levels as cardiovascular risk factors in coronary heart disease / Int. J. Cardiol 82:269~277
Finkelstein J / 1998 / The metabolism of homocysteine: pathways and regulation / Eur. J. Pediatr 157:S40~S44
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Selective Homocysteine Assay with Cucurbit[7]uril by pH Regulation
Journal of Microbiology and Biotechnology
2022 .04
-
Leukocyte Telomere Length is Associated With Serum Vitamin B12 and Homocysteine Levels in Older Adults With the Presence of Systemic Inflammation
Clinical Nutrition Research
2016 .01
-
Stabilization of Compact Protein Structures by Macrocyclic Hosts Cucurbit[n]urils in the Gas Phase
Mass Spectrometry Letters
2016 .03
-
A review: role of interfacial adhesion between carbon blacks and elastomeric materials
Carbon letters
2016 .01
-
CMAQ 모델의 화학메커니즘(SAPRC99, CB05) 적용에 따른 수도권 오존농도 모의결과 비교
한국환경과학회지
2016 .01
-
Poor nutrition and alcohol consumption are related to high serum homocysteine level at post-stroke
Nutrition Research and Practice
2015 .10
-
Poor nutrition and alcohol consumption are related to high serum homocysteine level at post-stroke
Nutrition Research and Practice
2015 .10
-
Tentative identification of phase I metabolites of CB-13, a novel synthetic cannabinoid by LC-TOF-MS
한국분석과학회 학술대회
2017 .05
-
Effects of excessive dietary methionine on oxidative stress and dyslipidemia in chronic ethanol-treated rats
Nutrition Research and Practice
2015 .04
-
Effects of excessive dietary methionine on oxidative stress and dyslipidemia in chronic ethanol-treated rats
Nutrition Research and Practice
2015 .04
-
A novel exopolysaccharide (p-CY01) from the Antarctic bacterium Pseudoalteromonas sp. Strain CY01 cryopreserves human red blood cells
극지연구소 초록집
2024 .07
-
Improvement of Electronic Properties and Amplification of Electron Trapping/Recovery through Liquid Crystal(LC) Passivation on Amorphous InGaZnO Thin Film Transistors
한국진공학회 학술발표회초록집
2016 .02
-
Simultaneous detection method of muscle developing five monoclonal antibodies in human plasma with using LC-MS/MS
한국분석과학회 학술대회
2023 .11
-
Effect of Terminal Labeling of Fluorescent Dyes on Ribozyme Activity
대한화학회지
2015 .08
-
Investigations of Temperature Effect on the Conduction Mechanism of Electrical Conductivity of Copolymer/Carbon Black Composite
한국현미경학회지
2017 .01
-
Detection of Carbon Dioxide Using Cucurbit[6]uril-Functionalized Gold Nanorod Gas Sensor Based on Localized Surface Plasmon Resonance
Applied Science and Convergence Technology
2021 .11
-
Validation study of in vitro potency test method using reporter gene for the therapeutic monoclonal antibodies
한국분석과학회 학술대회
2019 .05
-
Determining the Required Vitamin D Level for Bone Health Based on Bone Turnover Markers
대한임상검사과학회지
2017 .01
-
Variovorax terrae sp. nov. Isolated from Soil with Potential Antioxidant Activity
Journal of Microbiology and Biotechnology
2022 .07
-
Trends in Monoclonal Antibody Production Using Various Bioreactor Syst
Journal of Microbiology and Biotechnology
2021 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0