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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 426 - 439 (14page)
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Many small RNAs (sRNAs) regulate gene expression by base pairing to their target messenger RNAs (mRNAs) with the help of Hfq in Escherichia coli. The sRNA DsrA activates translation of the rpoS mRNA in an Hfq-dependent manner, but this activation ability was found to partially bypass Hfq when DsrA is overproduced. The precise mechanism by which DsrA bypasses Hfq is unknown. In this study, we constructed strains lacking all three rpoS-activating sRNAs (i.e., ArcZ, DsrA, and RprA) in hfq+ and Hfq- backgrounds, and then artificially regulated the cellular DsrA concentration in these strains by controlling its ectopic expression. We then examined how the expression level of rpoS was altered by a change in the concentration of DsrA. We found that the translation and stability of the rpoS mRNA are both enhanced by physiological concentrations of DsrA regardless of Hfq, but that depletion of Hfq causes a rapid degradation of DsrA and thereby decreases rpoS mRNA stability. These results suggest that the observed Hfq dependency of DsrA-mediated rpoS activation mainly results from the destabilization of DsrA in the absence of Hfq, and that DsrA itself contributes to the translational activation and stability of the rpoS mRNA in an Hfq-independent manner.
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참고문헌 (65)
참고문헌 신청
Andrade, J. M. / 2018 / The RNA-binding protein Hfq is important for ribosome biogenesis and affects translation fidelity / EMBO J 37:e97631
Andrade, J. M. / 2012 / The crucial role of PNPase in the degradation of small RNAs that are not associated with Hfq / RNA 18:844~855
Baba, T. / 2006 / Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants : the Keio collection / Mol. Syst. Biol 2:2006
Bak, G. / 2014 / Roles of rpoS-activating small RNAs in pathways leading to acid resistance of Escherichia coli / Microbiologyopen 3:15~28
Bandyra, K. J. / 2012 / The seed region of a small RNA drives the controlled destruction of the target mRNA by the endoribonuclease RNase E / Mol. Cell 47:943~953
Andrade, J. M. / 2012 / The crucial role of PNPase in the degradation of small RNAs that are not associated with Hfq / RNA 18:844~855
Baba, T. / 2006 / Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants : the Keio collection / Mol. Syst. Biol 2:2006
Bak, G. / 2014 / Roles of rpoS-activating small RNAs in pathways leading to acid resistance of Escherichia coli / Microbiologyopen 3:15~28
Bandyra, K. J. / 2012 / The seed region of a small RNA drives the controlled destruction of the target mRNA by the endoribonuclease RNase E / Mol. Cell 47:943~953
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