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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
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- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제29권 제3호
- 발행연도
- 2019.1
- 수록면
- 401 - 409 (9page)
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초록· 키워드
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Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of Escherichia coli can be enhanced by overexpressing ybeD, which was known to encode a hypothetical protein of unknown function. In the wild-type E. coli BL21(DE3), ybeD transcription level increased over five-fold when temperature was increased from 37°C to either 42°C or 46°C. To study the function of ybeD, a deletion strain and an overexpression strain were constructed. At 46°C, in comparison to the wild type, the ybeD-deletion reduced cell growth half-fold, and the ybeDoverexpression promoted cell growth over two-fold. The growth enhancement by ybeDoverexpression was much more pronounced at 46°C than 37°C. The ybeD-overexpression was also effective in other E. coli strains of MG1655, W3110, DH10B, and BW25113. These findings reveal that ybeD gene plays an important role in enduring high-temperature stress, and that ybeD-overexpression can be a prospective strategy to develop thermotolerant microbial hosts.
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참고문헌 (34)
참고문헌 신청
Ezemaduka AN / 2014 / A small heat shock protein enables Escherichia coli to grow at a lethal temperature of 50oC conceivably by maintaining cell envelope integrity / J. Bacteriol 196:2004~2011
Liu D / 2009 / Enhanced thermotolerance of E, coli by expressed OsHsp90 from rice (Oryza sativa L.) / Curr. Microbiol 58:129~133
Gasser B / 2008 / Protein folding and conformational stress in microbial cells producing recombinant proteins : a host comparative overview / Microb. Cell Fact 7:11
Zhang X / 2014 / Heat-shock response transcriptional program enables high-yield and high-quality recombinant protein production in Escherichia coli / ACS Chem. Biol 9:1945~1949
Arsene F / 2000 / The heat shock response of Escherichia coli / Int. J. Food Microbiol 55:3~9
Liu D / 2009 / Enhanced thermotolerance of E, coli by expressed OsHsp90 from rice (Oryza sativa L.) / Curr. Microbiol 58:129~133
Gasser B / 2008 / Protein folding and conformational stress in microbial cells producing recombinant proteins : a host comparative overview / Microb. Cell Fact 7:11
Zhang X / 2014 / Heat-shock response transcriptional program enables high-yield and high-quality recombinant protein production in Escherichia coli / ACS Chem. Biol 9:1945~1949
Arsene F / 2000 / The heat shock response of Escherichia coli / Int. J. Food Microbiol 55:3~9
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