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- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
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The main goal of this study was to determine the genetic diversity among 36 grape cultivars grown in Palestine by using ISSR-polymerase chain reaction (PCR) fingerprints. Among the tested primers, 17 produced reasonable amplification products with high intensity and pattern stability. A total of 57 DNA fragments (loci) separated by electrophoresis on agarose gels were detected and they ranged in size, from 150 to 900 bp. Out of these fragments, 55 (88%) were polymorphic and 2 (3.5%) monomorphic. Our results also revealed an average of 3.1 loci per primer. A minimum of 1 and maximum of 10 DNA fragments were obtained (S-17, #820 and #841) and (S-31) primers, respectively. Therefore, the later primer (S-31) is considered to be the most powerful primer among the tested ones. The genetic distance matrix showed an average distance range of between 0.05 and 0.76. The maximum genetic distance value of 0.76 (24% similarity) was exhibited between the (Shami and Marawi.Hamadani.Adi) as well as (Bairuti and Marawi.Hamadani.Adi) genotypes. On the other hand, the lowest genetic distance of 0.05 (95% similarity) was exhibited between (Jandali.Tawel.Mofarad and Jandali. Kurawi.Mlzlz) along with (Shami.Aswad and Shami.mtartash. mlwn) genotypes. Furthermore, the UPGMA dendrogram generally clusters the grape cultivars into eight major clusters in addition to an isolated genotype. Based on these figures, the cultivars tested in this study could be characterized by large divergence at the DNA level. This is taking the assumption that our region has a very rich and varied clonal grape genetic structure.
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참고문헌 (29)
참고문헌 신청
Achtak H / 2009 / Microsatellite Markers as Reliable Tools for Fig Cultivar Identification / J Amer Soc Hort. Sci 134(6):624~631
Agarwal M / 2008 / Advances in molecular marker techniques and their applications in plant sciences / Plant Cell Reports 27:617~631
Bahurupe J / 2013 / Genetic diversity analysis in chilli (Capsicum annuum L.) using RAPD markers / The international quarterly journal of life sciences 8(3):915~918
Basheer-Salimia R / 2012 / Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.) / American Journal of Plant Sciences 3(9):1241~1251
Basheer-Salimia R / 2015 / Ampelographic and phenotypic characterizations of white Palestinian grapevine cultivars / Palestine Technical University-Kadoorie Research Journal 3(1):1~11
Agarwal M / 2008 / Advances in molecular marker techniques and their applications in plant sciences / Plant Cell Reports 27:617~631
Bahurupe J / 2013 / Genetic diversity analysis in chilli (Capsicum annuum L.) using RAPD markers / The international quarterly journal of life sciences 8(3):915~918
Basheer-Salimia R / 2012 / Assessments of Biodiversity Based on Molecular Markers and Morphological Traits among West-Bank, Palestine Fig Genotypes (Ficus carica L.) / American Journal of Plant Sciences 3(9):1241~1251
Basheer-Salimia R / 2015 / Ampelographic and phenotypic characterizations of white Palestinian grapevine cultivars / Palestine Technical University-Kadoorie Research Journal 3(1):1~11
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