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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 139 - 147 (9page)
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Glutamate decarboxylase catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), contributing to pH homeostasis through proton consumption. The reaction is the first step toward the GABA shunt. To date, the enzymes involved in the glutamate metabolism of Photobacterium damselae subsp. piscicida have not been elucidated. In this study, an open reading frame of P. damselae subsp. piscicida, showing homology to the glutamate decarboxylase or putative pyridoxal-dependent aspartate 1-decarboxylase genes, was isolated and cloned into an expression vector to produce the recombinant enzyme. Preliminary gas chromatography-mass spectrometry characterization of the purified recombinant enzyme revealed that it catalyzed not only the decarboxylation of glutamate but also the transamination of GABA. This enzyme of P. damselae subsp. piscicida could be bifunctional, combining decarboxylase and transaminase activities in a single polypeptide chain.
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참고문헌 (44)
참고문헌 신청
Erlander MG / 1991 / The structural and functional heterogeneity of glutamic acid decarboxylase: a review / Neurochem. Res 16:215~226
Jakobs C / 1993 / Inherited disorders of GABA metabolism / J. Inherit. Metab. Dis 16:704~715
Ting Wong CG / 2003 / GABA, γ-hydroxybutyric acid, and neurological disease / Ann. Neurol 54:S3~S12
Park KB / 2007 / Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3 / Bioresour. Technol 98:312~319
Xu N / 2017 / Biotechnological advances and perspectives of gamma-aminobutyric acid production / World J. Microbiol. Biotechnol 33:1~11
Jakobs C / 1993 / Inherited disorders of GABA metabolism / J. Inherit. Metab. Dis 16:704~715
Ting Wong CG / 2003 / GABA, γ-hydroxybutyric acid, and neurological disease / Ann. Neurol 54:S3~S12
Park KB / 2007 / Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3 / Bioresour. Technol 98:312~319
Xu N / 2017 / Biotechnological advances and perspectives of gamma-aminobutyric acid production / World J. Microbiol. Biotechnol 33:1~11
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