지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 242 - 249 (8page)
이용수
초록· 키워드
오류제보하기
Biosynthesis of indole-3-acetate (IAA) from L-tryptophan via indole-3-pyruvate pathway requires three enzymes including aminotransferase, indole-3-pyruvate decarboxylase, and indole-3-acetate dehydrogenase. To establish a bio-based production of IAA, the aspC, ipdC, and iad1 from Escherichia coli, Enterobacter cloacae, and Ustilago maydis, respectively, were expressed under control of the tac, ilvC, and sod promoters in C. glutamicum. Cells harboring ipdC produced tryptophol, indicating that the ipdC product is functional in this host. Analyses of SDS-PAGE and enzyme activity revealed that genes encoding AspC and Iad1 were efficiently expressed from the sod promoter, and their enzyme activities were 5.8 and 168.5 nmol/ min/mg-protein, respectively. The final resulting strain expressing aspC, ipdC, and iad1 produced 2.3 g/l and 7.3 g/l of IAA from 10 g/l L-tryptophan, respectively, in flask cultures and a 5-L bioreactor.
목차
등록된 정보가 없습니다.
참고문헌 (21)
참고문헌 신청
Elisa Friska Romasi / 2013 / Development of Indole-3-Acetic Acid-Producing Escherichia coli by Functional Expression of IpdC, AspC, and Iad1 / Journal of Microbiology and Biotechnology 23(12):1726~1736
Sergeeva E / 2002 / Evidence for production of the phytohormone indole-3-acetic acid by cyanobacteria / Planta 215:229~238
Spaepen S / 2007 / Indole-3-acetic acid in microbial and microorganism-plant signaling / FEMS Microbiol. Rev 31:425~448
Spaepen S / 2011 / Auxin and plant-microbe interactions / Cold Spring Harb Perspect. Biol 3:1~13
Reineke G / 2008 / Indole-3-acetic acid (IAA) biosynthesis in the smut fungus Ustilago maydis and its relevance for increased IAA levels in infected tissue and host tumour formation / Mol. Plant Pathol 9:339~355
Sergeeva E / 2002 / Evidence for production of the phytohormone indole-3-acetic acid by cyanobacteria / Planta 215:229~238
Spaepen S / 2007 / Indole-3-acetic acid in microbial and microorganism-plant signaling / FEMS Microbiol. Rev 31:425~448
Spaepen S / 2011 / Auxin and plant-microbe interactions / Cold Spring Harb Perspect. Biol 3:1~13
Reineke G / 2008 / Indole-3-acetic acid (IAA) biosynthesis in the smut fungus Ustilago maydis and its relevance for increased IAA levels in infected tissue and host tumour formation / Mol. Plant Pathol 9:339~355
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Biosynthetic Pathway of Indole-3-Acetic Acid in Basidiomycetous Yeast Rhodosporidiobolus fluvialis
Mycobiology
2019 .01
-
인돌의 항균 효과에 의한 Pantoea agglomerans의 바이오필름 생성 억제 조절에 관한 연구
한국환경과학회지
2021 .05
-
반응표면분석법을 활용한 Pantoea agglomerans SRCM 119864의 Indole-3-acetic acid 생산 배지 최적화
생명과학회지
2022 .11
-
Isolation of Indole-3-Acetic Acid (IAA) Producing Arthrobacter Species and Study on Their Plant Growth Effects
한국미생물학회 학술대회논문집
2022 .10
-
Indole Induce the Degradation of Anthranilate in Pseudomonas
한국미생물학회 학술대회논문집
2015 .04
-
Phylogenetic and Genomic Analysis of Indole Acetic Acid (IAA)-producing Stenotrophomonas sp. nov. FBCC-B152 from Fresh Water
한국미생물학회 학술대회논문집
2022 .10
-
Application of Indole-3-Acetic Acid (IAA) derived from Sphingomonas sp. CV7422 in the Cauliflower Mosaic Virus (CaMV) 35S Promoter Transgenic Microalga
한국미생물학회 학술대회논문집
2022 .10
-
Applications of Quorum Sensing Molecule Indole to Control Bacterial Virulence and Host Immunity
한국미생물학회 학술대회논문집
2017 .04
-
Draft genome sequence of Tianweitania sediminis isolated from the subsurface sediment core
미생물학회지
2021 .09
-
Production of Indole-3-Acetic Acid by Enterobacter sp. DMKU-RP206 Using Sweet Whey as a Low-Cost Feed Stock
Journal of Microbiology and Biotechnology
2018 .01
-
Establishment of simultaneous analysis method for Indole acetic acid, Abscisic acid, and Jasmonic acid in bacteria using HPLC-MS/MS
한국분석과학회 학술대회
2021 .11
-
Development of a Simultaneous Analytical Method for Indole Alkaloids in Foods using Liquid Chromatography-Mass Spectrometry
한국분석과학회 학술대회
2023 .11
-
Exogenous Indole Regulates Lipopeptide Biosynthesis in Antarctic Bacillus amyloliquefaciens Pc3
Journal of Microbiology and Biotechnology
2018 .01
-
Simultaneous determination of indole alkaloids by UPLC-ESI-Q-TOF and its application to medicinal plant
한국분석과학회 학술대회
2017 .11
-
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells
Nutrition Research and Practice
2023 .08
-
IPA and its precursors differently modulate the proliferation, differentiation, and integrity of intestinal epithelial cells
Nutrition Research and Practice
2023 .08
-
Conjugated polymers based on isoindigo(ID) and 6-(2-thienyl)-4H-thieno[3,2-b]indole(TTI) for organic solar cells
한국진공학회 학술발표회초록집
2017 .02
-
The Role of Pyruvate Metabolism in Mitochondrial Quality Control and Inflammation
Molecules and Cells
2023 .05
-
Indole-induced Activities of β-Lactamase and Efflux Pump Confer Ampicillin Resistance in Pseudomonas putida KT2440
한국미생물학회 학술대회논문집
2017 .04
-
Production of Exopolysaccharides and İndole Acetic Acid (IAA) by Rhizobacteria and Their Potential against Drought Stress in Upland Rice
Journal of Microbiology and Biotechnology
2024 .06
이 논문의 저자 정보
최근 본 자료
댓글(0)
0