지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.12
- 수록면
- 581 - 585 (5page)
이용수
초록· 키워드
오류제보하기
Confocal laser scanning microscopy (CLSM) was used to examine archaeoparasitological specimens from coprolites associated with La Cueva de los Muertos Chiquitos (CMC) located near present-day Durango, Mexico. The eggs for 4 different types of parasites recovered from CMC coprolites were imaged using CLSM to assist with identification efforts. While some of the parasite eggs recovered from CMC coprolites were readily identified using standard light microscopy (LM), CLSM provided useful data for more challenging identifications by highlighting subtle morphological features and enhancing visualization of parasite egg anatomy. While other advanced microscopy techniques, such as scanning electron microscopy (SEM), may also detect cryptic identifying characters, CLSM is less destructive to the specimens. Utilizing CLSM allows for subsequent examinations, such as molecular analyses, that cannot be performed following SEM sample preparation and imaging. Furthermore, CLSM detects intrinsic autofluorescence molecules, making improved identification independent of resource and time-intensive protocols. These aspects of CLSM make it an excellent method for assisting in taxonomic identification and for acquiring more detailed images of archaeoparasitological specimens.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (13)
참고문헌 신청
Böhm A / 2011 / Confocal imaging of the exo-and endoskeleton of Protura after nondestructive DNA extraction / Soil Organisms 83:335~345
Chetverikoc PE / 2012 / Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites(Acari : Eriophyoidea) / Zootaxa 3453:56~68
Grzywacz A / 2014 / Confocal laser scanning microscopy as a valuable tool in Diptera larval morphology studies / Parasitol Res 113:4297~4302
Klaus AV / 2006 / Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods(Insecta) / Integr Comp Biol 46:207~214
Lee S / 2009 / Use of confocal laser scanning microscopy in systematics of insects with a comparison of fluorescence from different stains / Syst Entomol 34:10~14
Chetverikoc PE / 2012 / Confocal laser scanning microscopy technique for the study of internal genitalia and external morphology of eriophyoid mites(Acari : Eriophyoidea) / Zootaxa 3453:56~68
Grzywacz A / 2014 / Confocal laser scanning microscopy as a valuable tool in Diptera larval morphology studies / Parasitol Res 113:4297~4302
Klaus AV / 2006 / Novel methodology utilizing confocal laser scanning microscopy for systematic analysis in arthropods(Insecta) / Integr Comp Biol 46:207~214
Lee S / 2009 / Use of confocal laser scanning microscopy in systematics of insects with a comparison of fluorescence from different stains / Syst Entomol 34:10~14
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UCI(KEPA) : I410-ECN-0101-2020-513-000264462