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논문 기본 정보

자료유형
학술저널
저자정보
박한울 (인하대학교) 강성모 (인하대학교) 민지호 (인하대학교) 김준호 (인하대학교) 조용희 (인하대학교) 김기현 (인하대학교) 진언선 (한양대학교) 홍성주 (인하대학교) 이철균 (인하대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.35 No.1(Wn.172)
발행연도
2020.3
수록면
78 - 83 (6page)
DOI
10.7841/ksbbj.2020.35.1.78

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초록· 키워드

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Beside the commercialized pigments from microalgae, such as astaxanthin, beta-carotene, and c-phycocyanin, zeaxanthin is another pigment with great potential for commercialization as it can be used to prevent or cure age-related macular degeneration. However, low zeaxanthin productivity due to its low content in microalgal biomass has been the major obstacle to commercialization. With recent advances in genome editing technology, a green microalga Chlamydomonas reinhardtii dZL was engineered to accumulate zeaxanthin with growth by knocking out enzymes that convert zeaxanthin into other carotenoids. In the present study, C. reinhardtii dZL was cultivated using TAP medium in 100 L flat-panel photobioreactors (PBRs), and the effect of initial cell density(ICD) on biomass productivity in batch operation was investigated. ICD of 0.1 g/L was predicted to yield highest biomass productivity from experiments using 2 L bubble column PBRs. In 100 L flat-panel PBRs, as ICD increased from 0.005 g/L to 0.08 g/L, biomass productivity was increased from 0.03 g/L/ day to 0.16 g/L/day. However, further increasing ICD from 0.08 g/L to 0.16 g/L reduced to 0.14 g/L/day as the maximum biomass concentration was limited by the light intensity. The results showed that ICD could affect biomass productivity in positive and negative ways, and thus ICD should carefully be determined with regards to the culture conditions. Such strategy could be applied to improve productivity for other compounds of interests in other microalgae as well.

목차

Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2020-470-000539360