지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.7
- 수록면
- 570 - 579 (10page)
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초록· 키워드
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Background: Many researchers reported that the various immune activities of red ginseng are due to acid polysaccharides. But, the exact structural characteristics of the acidic polysaccharide in red ginseng have not been fully elucidated. Therefore, we isolated the acidic polysaccharide from red ginseng and characterized the structural property of the active moiety of this polysaccharide, which contributes to the immunostimulatory activity of red ginseng.
Methods: A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100. Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity. The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis.
Results: Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α. The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis. RGPAP- I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%). RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan. Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
Conclusion: we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(→/4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β- 3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
Methods: A polysaccharide (RGP-AP-I) was purified from red ginseng via size-exclusion chromatography using Sephadex G-100. Immunostimulatary activity of RGP-AP-I was investigated via anti-complementory and macrophage stimulatory activity. The structure of RGP-AP-I was characterized by HPLC, sugar composition, β-glucosyl Yariv reagent and methylation analysis.
Results: Peritoneal macrophages stimulated using RGP-AP-I significantly augmented the production of various cytokines such as interleukin (IL)-6, IL-12, and tumor necrosis factor (TNF)-α. The primary structure of RGP-AP-I was elucidated by assessing its sugar composition and methylation analysis. RGPAP- I is a 96 kDa acidic polysaccharide, and comprises nine different monosaccharides, which mainly include sugars such as rhamnose (Rha, 9.5%), galacturonic acid (GalA, 18.4%), galactose (Gal, 30.4%), and arabinose (Ara, 35.0%). RGP-AP-I exhibited an considerable reaction with the β-glucosyl Yariv reagent, revealing the presence of arabino-β-3,6-galactan. Methylation analysis indicated that RGP-AP-I comprises 21 different glycosyl linkages, such as 3-, 4-, 6- and 3,6-linked Galp; 5-linked Araf; 2,4-linked Rhap; and 4-linked GalAp, which are characteristics of rhamnogalacturonan I (RG-I).
Conclusion: we assumed that the immunostimulatory activity of RGP-AP-I may be due to the RG-I structure, which comprises a main chain with a repeating linkage unit, [→2)-Rhap-(→/4)-GalAp-(1→] and three groups of side chains such as (1→5)-linked arabinan, (1→4)-linked galactan, and arabino-β- 3,6-galactan, which branch at the C(O)4 positions of Rha residues in the main chain of RGP-AP-I.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
4. Conclusion
References
참고문헌 (39)
참고문헌 신청
Byeon SE / 2012 / Molecular mechanism of macrophage activation by red ginseng acidic polysaccharide from Korean red ginseng / Mediat Inflamm 732860:1~7
김종훈 / 2012 / Cardiovascular Diseases and Panax ginseng: A Review on Molecular Mechanisms and Medical Applications / Journal of Ginseng Research 36(1):16~26
Wong AS / 2015 / Recent advances in ginseng as cancer therapeutics : a functional andmechanistic overview / Nat Prod Reports 32:256~272
원해단 / 2012 / Ginseng and Diabetes: The Evidences from In Vitro, Animal and Human Studies / Journal of Ginseng Research 36(1):27~39
Kyung Hee Lee / 2016 / Antihypertensive effect of Korean Red Ginseng by enrichment of ginsenoside Rg3 and arginineefructose / Journal of Ginseng Research 40(3):237~244
김종훈 / 2012 / Cardiovascular Diseases and Panax ginseng: A Review on Molecular Mechanisms and Medical Applications / Journal of Ginseng Research 36(1):16~26
Wong AS / 2015 / Recent advances in ginseng as cancer therapeutics : a functional andmechanistic overview / Nat Prod Reports 32:256~272
원해단 / 2012 / Ginseng and Diabetes: The Evidences from In Vitro, Animal and Human Studies / Journal of Ginseng Research 36(1):27~39
Kyung Hee Lee / 2016 / Antihypertensive effect of Korean Red Ginseng by enrichment of ginsenoside Rg3 and arginineefructose / Journal of Ginseng Research 40(3):237~244
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UCI(KEPA) : I410-ECN-0101-2020-524-000843275