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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제30권 제5호
- 발행연도
- 2020.1
- 수록면
- 785 - 792 (8page)
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초록· 키워드
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L-Theanine, found in green tea leaves has been shown to positively affect immunity and relaxation in humans. There have been many attempts to produce L-theanine through enzymatic synthesis to overcome the limitations of traditional methods. Among the many genes coding for enzymes in the L-theanine biosynthesis, glutamylmethylamide synthetase (GMAS) exhibits the greatest possibility of producing large amounts of production. Thus, GMAS from Methylovorus mays No. 9 was overexpressed in several strains including vectors with different copy numbers. BW25113(DE3) cells containing the pET24ma::gmas was selected for strains. The optimal temperature, pH, and metal ion concentration were 50oC, 7, and 5 mM MnCl2, respectively. Additionally, ATP was found to be an important factor for producing high concentration of L-theanine so several strains were tested during the reaction for ATP regeneration. Baker’s yeast was found to decrease the demand for ATP most effectively. Addition of potassium phosphate source was demonstrated by producing 4-fold higher L-theanine. To enhance the conversion yield, GMAS was additionally overexpressed in the system. A maximum of 198 mM L-theanine was produced with 16.5 mmol/l/h productivity. The whole-cell reaction involving GMAS has greatest potential for scale-up production of L-theanine.
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참고문헌 (41)
참고문헌 신청
Zheng G / 2004 / Anti-obesity effects of three major components of green tea, catechins, caffeine and theanine, in mice / In Vivo 18:55~62
Turkozu D / 2017 / L-theanine, unique amino acid of tea, and its metabolism, health effects, and safety / Crit. Rev. Food Sci. Nutr 57:1681~1687
Saeed M / 2017 / Green tea(Camellia sinensis)and l-theanine : Medicinal values and beneficial applications in humans-A comprehensive review / Biomed. Pharmacother 95:1260~1275
Adhikary R / 2017 / L-theanine : A potential multifaceted natural bioactive amide as health supplement / Asian Pac. J. Trop. Biomed 7:842~848
Vuong QV / 2011 / Optimum conditions for the water extraction of L-theanine from green tea / J. Sep. Sci 34:2468~2474
Turkozu D / 2017 / L-theanine, unique amino acid of tea, and its metabolism, health effects, and safety / Crit. Rev. Food Sci. Nutr 57:1681~1687
Saeed M / 2017 / Green tea(Camellia sinensis)and l-theanine : Medicinal values and beneficial applications in humans-A comprehensive review / Biomed. Pharmacother 95:1260~1275
Adhikary R / 2017 / L-theanine : A potential multifaceted natural bioactive amide as health supplement / Asian Pac. J. Trop. Biomed 7:842~848
Vuong QV / 2011 / Optimum conditions for the water extraction of L-theanine from green tea / J. Sep. Sci 34:2468~2474
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