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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 323 - 330 (8page)
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초록· 키워드
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The high expression level of industrial and metabolically important proteins in plants can be achieved by plastid transformation. The CaIA vector, a Capsicum- specific vector harboring aadA (spectinomycin resistance), is a selectable marker controlled by the PsbA promoter, and the terminator is flanked by the trnA and trnI regions of the inverted repeat (IR) region of the plastid. The CaIA vector can introduce foreign genes into the IR region of the plastid genome. The biolistic method was used for chloroplast transformation in Scoparia dulcis with leaf explants followed by antibiotic selection on regeneration medium. Transplastomes were successfully screened, and the trans-formation efficiency of 3 transgenic lines from 25 bombarded leaf explants was determined. Transplastomic lines were evaluated by PCR and Southern blotting for the confirmation of aadA insertion and its integration into the chloroplast genome. Seeds collected from transplastomes were analyzed on spectinomycin medium with wild types to determine genetic stability. The increased chloroplast transformation efficiency (3 transplastomic lines from 25 bombarded explants) would be useful for expressing therapeutically and industrially important genes in Scoparia dulcis L.
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등록된 정보가 없습니다.
참고문헌 (51)
참고문헌 신청
Mahender Aileni / 2011 / Highly efficient production of transgenic Scoparia dulcis L. mediated by Agrobacterium tumefaciens: plant regeneration via shoot organogenesis / Plant Biotechnology Reports 5(2):147~156
Aileni M / 2011 / In Vitro Regeneration, Flowering and Seed Formation from Leaf Explants of Scoparia dulcis L
Bock R / 1995 / In vivo testing of a tobacco plastid DNA segment for guide RNA function in psbL editing / Molecular and General Genetics MGG 247:439~443
Boynton JE / 1988 / Chloroplast transformation in Chlamydomonas with high velocity microprojectiles / Science 240:1534~1538
Cheng L / 2010 / Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons / Plant cell reports 29:371~381
Aileni M / 2011 / In Vitro Regeneration, Flowering and Seed Formation from Leaf Explants of Scoparia dulcis L
Bock R / 1995 / In vivo testing of a tobacco plastid DNA segment for guide RNA function in psbL editing / Molecular and General Genetics MGG 247:439~443
Boynton JE / 1988 / Chloroplast transformation in Chlamydomonas with high velocity microprojectiles / Science 240:1534~1538
Cheng L / 2010 / Chloroplast transformation of rapeseed (Brassica napus) by particle bombardment of cotyledons / Plant cell reports 29:371~381
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